公开(公告)号：US20190153391A1

公开(公告)日：2019-05-23

申请号：US15511275

申请日：2015-09-14

Applicant: OSAKA UNIVERSITY

Inventor： Yukiko OCHI ,  Kiyotoshi SEKIGUCHI ,  Shigeru MIYAGAWA ,  Yoshiki SAWA ,  Antti Markus SILTANEN

IPC: C12N5/077 ,  C12N5/074 ,  A61K35/34

CPC classification number: C12N5/0657 ,  A61K35/34 ,  C12N5/0696 ,  C12N2506/45 ,  C12N2533/52

Abstract: The present invention provides a method for preparing a clinically applicable, safe and less damaged cardiomyocyte population through a brief and simple procedure from a cell population obtained by induced differentiation of pluripotent stem cells into cardiomyocytes. The present invention relates to a method for preparing a cardiomyocyte population, the method comprising the steps of: (1) inducing pluripotent stem cells to differentiate into cardiomyocytes, (2) bringing a cell population obtained by the induced differentiation into contact with a laminin selected from the group consisting of laminin α2β1γ1, laminin α2β2γ1, laminin α1β1γ1 and laminin α1β2γ1, or a fragment thereof having integrin binding activity, and (3) retrieving cells adherent to the laminin or the laminin fragment.