公开(公告)号：US20220213540A1

公开(公告)日：2022-07-07

申请号：US17605640

申请日：2020-04-22

Applicant: POSTECH RESEARCH AND BUSINESS DEVELOPMENT FOUNDATION

Inventor： Jeong Wook LEE ,  Gyoo Yeol JUNG ,  Chang Ha WOO ,  Sungho Jang ,  Giyoung SHIN

IPC: C12Q1/6862 ,  C12Q1/6876

Abstract: The present invention relates to a novel probe set for an isothermal one-pot reaction, and uses thereof and, particularly, provides a method for easily, accurately, and quickly diagnosing molecules, in particular, infection diseases, in the field, the method having a form applicable in the field on the basis of a nucleic acid sequence by using a one-pot reaction composition under an isothermal condition. If a molecular diagnostic platform according to the present invention is used, a target sequence can be quickly and accurately detected. Also, elements (reaction buffer, enzyme) needed in a diagnostic process using the present invention are much more simple and convenient than in a conventional antibody-based diagnosis, and, thus, the diagnostic process can be performed by a non-skilled person, and the sensitivity and speed of diagnosis can be increased, as all reactions are unified at a constant temperature without expensive equipment used in general nucleic-acid-based molecular diagnostic technology, and an amplification process is performed automatically during a reaction process.

公开(公告)号：US20250092451A1

公开(公告)日：2025-03-20

申请号：US18967805

申请日：2024-12-04

Applicant: POSTECH RESEARCH AND BUSINESS DEVELOPMENT FOUNDATION

Inventor： Jeong Wook LEE ,  Gyoo Yeol JUNG ,  Chang Ha WOO ,  Sungho Jang ,  Giyoung SHIN

IPC: C12Q1/6862 ,  C12Q1/6832 ,  C12Q1/6876

Abstract: The present invention relates to a novel probe set for an isothermal one-pot reaction, and uses thereof and, particularly, provides a method for easily, accurately, and quickly diagnosing molecules, in particular, infection diseases, in the field, the method having a form applicable in the field on the basis of a nucleic acid sequence by using a one-pot reaction composition under an isothermal condition. If a molecular diagnostic platform according to the present invention is used, a target sequence can be quickly and accurately detected. Also, elements (reaction buffer, enzyme) needed in a diagnostic process using the present invention are much more simple and convenient than in a conventional antibody-based diagnosis, and, thus, the diagnostic process can be performed by a non-skilled person, and the sensitivity and speed of diagnosis can be increased, as all reactions are unified at a constant temperature without expensive equipment used in general nucleic-acid-based molecular diagnostic technology, and an amplification process is performed automatically during a reaction process.

公开(公告)号：US20250163408A1

公开(公告)日：2025-05-22

申请号：US18743720

申请日：2024-06-14

Applicant: POSTECH RESEARCH AND BUSINESS DEVELOPMENT FOUNDATION

Inventor： Jeong Wook LEE ,  Yun-nam CHOI ,  Dong Hyeon KIM ,  Seong Beom LEE

IPC: C12N15/11 ,  C12N1/20 ,  C12N9/00

Abstract: The present invention relates to a genetic biocontainment technology to prevent theft or misuse of biological resources (e.g., nucleic acid molecule, plasmid or microbial strain containing the nucleic acid molecule, or sequence information associated with the nucleic acid molecule) using a quadruplet codon and decoding system thereof. In particular, the present invention relates to a system or a microbial strain comprising a nucleic acid molecule in which a triplet codon encoding a specific amino acid in a nucleic acid sequence has been replaced with a quadruplet codon, wherein genetic biocontainment occurs in an environment where tRNA for the quadruplet codon does not exist, and a method using the same. The present invention also relates to a biocontainment method to protect sequence information using a quadruplet codon.

公开(公告)号：US20250051829A1

公开(公告)日：2025-02-13

申请号：US18717794

申请日：2022-12-08

Applicant: POSTECH Research and Business Development Foundation

Inventor： Chang Ha WOO ,  Jeong Wook LEE ,  Jeongmin LEE ,  Doeon SUNG

IPC: C12Q1/6816 ,  C12Q1/6888

Abstract: The present invention relates to a method for generating a single-stranded region for detecting genetic mutation and a method for detecting genetic mutation using same. A detection method according to the present invention can detect sequence-specific nucleic acids and detect mutations at the single nucleotide polymorphism (SNP) discrimination level, thereby allowing for the accurate and sensitive discrimination of mutations in a short time. Thus, the present invention provides an effective mutation detection method that can respond not only to current SARS-CoV-2 variants but also generally to specific diseases or related mutations in the future.

公开(公告)号：US20240376553A1

公开(公告)日：2024-11-14

申请号：US18658036

申请日：2024-05-08

Applicant: POSTECH Research and Business Development Foundation

Inventor： Jeong Wook LEE ,  Mi Bang KIM ,  Ye Rim SHIN ,  Chang Ha WOO

IPC: C12Q1/689 ,  C12Q1/682

Abstract: The present disclosure relates to a probe set for isothermal one-pot reaction for detecting strains with biologically active biosynthetic pathway and uses thereof. The platform according to the present disclosure selectively binds only to the functional metabolite essential biosynthetic gene cluster by using the probe set according to the present disclosure, and thus it is possible to perform phenotype-based screening on strains isolated using the probe set according to the present disclosure, thereby effectively screening strains with biologically biosynthetic pathway (functional metabolite biosynthetic strains) in a fast and accurate manner. Furthermore, the platform according to the present disclosure is able to utilize RNA as well as DNA as targets, thereby detecting gene clusters that are actually actively expressed.