公开(公告)号：US20230243859A1

公开(公告)日：2023-08-03

申请号：US18054326

申请日：2022-11-10

Applicant: Pattern Bioscience, Inc.

Inventor： Chueh-Yu WU

IPC: G01N35/08 ,  B01L3/00

CPC classification number: G01N35/08 ,  B01L3/502784 ,  B01L3/502738 ,  B01L2300/0861 ,  B01L2200/16

Abstract: A microfluidic chip can include a microfluidic network that comprises a port, one or more test volumes, and one or more channels through which fluid must flow from the port to the test volume(s). A crosslinkable material can also be disposed within the microfluidic network such that the crosslinkable material is flowable through the channel(s). The crosslinkable material of the microfluidic chip may be exposed to light and/or heat to crosslink the material within and thereby occlude the channel(s). A method of loading the microfluidic chip can include disposing a liquid within a port of a microfluidic network that includes one or more test volumes and one or more channels; flowing each of one or more portions of the liquid from the port, through at least one of the channel(s), and into a respective one of the test volume(s); and directing a crosslinkable material into at least one of the channel(s) and cross-linking the crosslinkable material such that none of the test volume(s) are in fluid communication with the port when the portion(s) of the liquid are in the test volume(s).