公开(公告)号：US12128110B2

公开(公告)日：2024-10-29

申请号：US16648636

申请日：2018-09-19

Applicant: Paul Scherrer Institut

Inventor： Philipp Spycher ,  Roger Schibli ,  Martin Behe ,  Jori Wehrmuller

IPC: C07K16/32 ,  A61K47/68 ,  C07K16/28 ,  C12P21/00

CPC classification number: A61K47/6855 ,  A61K47/6803 ,  A61K47/6889 ,  C07K16/2878 ,  C07K16/32 ,  C12P21/00 ,  C07K2317/24 ,  C07K2317/41

Abstract: The present invention relates to a method for generating an antibody-payload conjugate by means of a microbial transglutaminase (MTG). The method comprises a step of conjugating a linker having a primary amine residue, said linker having the peptide structure (shown in N->C direction) (Aax)m-(Aax)(NH2)-(Aax)n-B-(Aax)o, or (Aax)m-B-(Aax)n-(Aax)(NH2)-(Aax)o, to a Gln residue comprised in the heavy or light chain of an antibody. Aax(NH2) is an amino acid, amino acid derivative or amino acid mimetic comprising a side chain having a primary amine group.