公开(公告)号：US20240139742A1

公开(公告)日：2024-05-02

申请号：US18559099

申请日：2022-05-02

Applicant: Paul Scherrer Institut

Inventor： Thomas Mortelmans ,  Yasin Ekinci ,  Xiaodan Li

IPC: B01L3/00 ,  G01N33/543

CPC classification number: B01L3/502761 ,  G01N33/54366 ,  B01L2400/0406

Abstract: The planetary outbreak of COVID-19 has led to a substantial death toll and has hindered the functioning of modern society. This underlines the importance of accurate, cost-effective serological antibody tests and point-of-care diagnostics to monitor the viral spread and to contain pandemics and endemics. This requires a cost-effective three-dimensional nanofluidic device for rapid and multiplexed detection of viral antibodies. The device is configured to size-dependently immobilize particles at predefined positions from a multiparticle mixture, giving rise to distinct trapping lines. It is shown with the device that distinctive lines can be used as an on-chip and on-bead fluorescent linked immunosorbent assay for the detection of immunoglobulin G (IgG) antibodies against the receptor-binding domain of SARS-CoV-2 in human serum. Device versatility is exhibited by on-bead color multiplexing for simultaneous detection of IgG and IgM in convalescent human serum and by concurrent detection of anti-spike (SARS-CoV-2) and anti-hemagglutinin (Influenza A) antibodies.