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1.发明申请公开(公告)号：US20230013253A1
公开(公告)日：2023-01-19
申请号：US17756951
申请日：2020-12-16
Applicant: Pfizer Inc.
Inventor： Saurabh Kant Bhardwaj , Chia Hung Chu
IPC: C12N15/86
Abstract: Provided are compositions and methods for stabilizing a transfection cocktail containing DNA-cationic polymer complexes for an extended time, while maintaining high transfection efficiency. Such stabilized transfection cocktail can be used to generate transfected cells that can produce, for example, rAAV vectors on a large scale without impacting the key attributes of the virus production, such as, titer, DNA packaged rAAV particle fraction, and rAAV vector purification profile.
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