公开(公告)号：US20240043869A1

公开(公告)日：2024-02-08

申请号：US18255868

申请日：2021-12-20

Applicant: PFIZER INC.

Inventor： William S KISH ,  John R. LIGHTHOLDER ,  Matthew K. ROACH ,  Tamara ZEKOVIC

IPC: C12N15/86 ,  B01D15/20 ,  B01D15/42 ,  B01D15/38 ,  B01D15/16

CPC classification number: C12N15/86 ,  B01D15/203 ,  B01D15/426 ,  B01D15/3823 ,  B01D15/166 ,  C12N2750/14143 ,  C12N2750/14151

Abstract: The present disclosure provides methods for purifying a recombinant AAV (rAAV) vector from a solution by affinity chromatography to produce an eluate enriched for AAV vectors (rAAV vectors).

公开(公告)号：US20240011012A1

公开(公告)日：2024-01-11

申请号：US18255869

申请日：2021-12-17

Applicant: PFIZER INC.

Inventor： Neha KALLA ,  William KISH ,  John LIGHTHOLDER ,  Zhuo LIU ,  Eric VORST ,  Tamara ZEKOVIC

IPC: C12N15/10 ,  C12N15/86

CPC classification number: C12N15/1003 ,  C12N15/86 ,  C12N2750/14143

Abstract: The present disclosure describes improved methods for use in purifying biological products made by host cells. In some embodiments, the improved methods comprise one or more steps of lysing host cells, such as with a detergent, to release the biological product, precipitating host cell DNA, such as with domiphen bromide, and then inhibiting precipitation of residual host cell DNA in a supernatant containing the biological product by adding a salt to a sufficient final concentration. In some embodiments, the biological product is a vaccine, or a viral vector for gene therapy, such as an AAV vector or a lentiviral vector.

公开(公告)号：US20230399656A1

公开(公告)日：2023-12-14

申请号：US18250252

申请日：2021-11-01

Applicant: Pfizer Inc.

Inventor： Alexander BERRILL ,  William S. KISH ,  John R. LIGHTHOLDER ,  Matthew K. ROACH ,  William B. WELLBORN ,  Tamara ZEKOVIC

IPC: C12N15/86 ,  B01D15/36 ,  B01D15/42 ,  B01D15/16

CPC classification number: C12N15/86 ,  B01D15/363 ,  B01D15/424 ,  B01D15/166 ,  C12N2750/14151 ,  C12N2750/14143

Abstract: The present disclosure provides methods for purifying a recombinant AAV (rAAV) vector from a solution by anion-exchange chromatography (AEX) to produce an eluate enriched for full capsids and depleted of empty capsids.