公开(公告)号：US20250147024A1

公开(公告)日：2025-05-08

申请号：US18505075

申请日：2023-11-08

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Simon Jochum ,  Matthias Hemann ,  Juliane Benz

IPC: G01N33/569 ,  C12N9/10 ,  G01N33/68

Abstract: Fusion polypeptides are disclosed which are substrates for Kutzneria albida transglutaminase. The fusion polypeptides comprise one or more FKBP chaperone(s) and a target polypeptide. Each of these elements is separated from the neighboring element by a linker amino acid sequence. It was found that inserting glutamic acid containing transglutaminase recognition motifs into the linker amino acid chains is advantageous. Subsequent labeling reactions catalyzed by the transglutaminase surprisingly provide labeled fusion polypeptides have superior properties when compared with chemically random-labeled fusion polypeptides of similar design. Assays and kits are provided for in vitro detection of target antibodies in samples.

公开(公告)号：US20210333277A1

公开(公告)日：2021-10-28

申请号：US16856162

申请日：2020-04-23

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Alexander Riedel ,  Christian Scholz ,  Peter Muench ,  Juliane Benz ,  Markus Eckl ,  Frederik Baumkoetter

IPC: G01N33/569 ,  C12N7/00

Abstract: The present invention relates to a Corona antigen comprising a Corona nucleocapsid specific amino acid sequence, compositions, and reagent kits comprising the same and methods of producing it. Also encompassed are methods of detecting anti-Corona antibodies in samples using said Corona antigen, and methods of differential diagnosis of an immune response in a patient due to natural Corona infection or due to vaccination against Corona.

公开(公告)号：US20250155435A1

公开(公告)日：2025-05-15

申请号：US18506002

申请日：2023-11-09

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Simon Jochum ,  Matthias Hemann ,  Juliane Benz

IPC: G01N33/569 ,  C07K14/005 ,  C12N9/10 ,  G01N33/532

Abstract: Fusion polypeptides are disclosed which are substrates for Kutzneria albida transglutaminase. The fusion polypeptides comprise one or more FKBP chaperone(s) and a target polypeptide. Each of these elements is separated from the neighboring element by a linker amino acid sequence. It was found that inserting glutamic acid containing transglutaminase recognition motifs into the linker amino acid chains is advantageous. Subsequent labeling reactions catalyzed by the transglutaminase surprisingly provide labeled fusion polypeptides have superior properties when compared with chemically random-labeled fusion polypeptides of similar design. Assays and kits are provided for in vitro detection of target antibodies in samples.

公开(公告)号：US11319351B2

公开(公告)日：2022-05-03

申请号：US16663457

申请日：2019-10-25

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Elke Faatz ,  Alexander Riedel ,  Christian Scholz ,  Peter Muench ,  Gloria Tabares ,  Mario Gloeck ,  Silke Luebcke ,  Juliane Benz

IPC: C07K14/005 ,  C07K14/18 ,  C12N7/00 ,  G01N33/569

Abstract: The disclosure relates to a polypeptide suitable for detecting antibodies against a flavivirus in an isolated biological sample having a flavivirus NS1 wing domain specific amino acid sequence, wherein no amino acid sequences from the NS1 ß-ladder domain of said flavivirus are present in the polypeptide. In an embodiment, the flavivirus is selected from Zika virus (ZIKV), West-Nile virus (WNV), Dengue virus types 1-4 (DENV1-4), tick-borne encephalitis virus (TBEV), yellow fever virus (YFV) and Japanese encephalitis virus (JEV). Also disclosed is a method for producing said flaviviral NS1 wing domain specific polypeptides, a method for detecting antibodies specific for a first flavivirus species, the use of said flaviviral NS1 wing domain specific polypeptides for detecting antibodies as well as a reagent kit for detecting said flavivirus antibodies that has a flavivirus NS1 wing domain polypeptide.

公开(公告)号：US20200048313A1

公开(公告)日：2020-02-13

申请号：US16663457

申请日：2019-10-25

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Elke Faatz ,  Alexander Riedel ,  Christian Scholz ,  Peter Muench ,  Gloria Tabares ,  Mario Gloeck ,  Silke Luebcke ,  Juliane Benz

IPC: C07K14/18 ,  G01N33/569 ,  C12N7/00

Abstract: The disclosure relates to a polypeptide suitable for detecting antibodies against a flavivirus in an isolated biological sample having a flavivirus NS1 wing domain specific amino acid sequence, wherein no amino acid sequences from the NS1 ß-ladder domain of said flavivirus are present in the polypeptide. In an embodiment, the flavivirus is selected from Zika virus (ZIKV), West-Nile virus (WNV), Dengue virus types 1-4 (DENV1-4), tick-borne encephalitis virus (TBEV), yellow fever virus (YFV) and Japanese encephalitis virus (JEV). Also disclosed is a method for producing said flaviviral NS1 wing domain specific polypeptides, a method for detecting antibodies specific for a first flavivirus species, the use of said flaviviral NS1 wing domain specific polypeptides for detecting antibodies as well as a reagent kit for detecting said flavivirus antibodies that has a flavivirus NS1 wing domain polypeptide.

公开(公告)号：US12018053B2

公开(公告)日：2024-06-25

申请号：US17705704

申请日：2022-03-28

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Elke Faatz ,  Alexander Riedel ,  Christian Scholz ,  Peter Muench ,  Gloria Tabares ,  Mario Gloeck ,  Silke Luebcke ,  Juliane Benz

IPC: C07K14/18 ,  C07K14/005 ,  C12N7/00 ,  G01N33/569

CPC classification number: C07K14/1825 ,  C07K14/005 ,  C12N7/00 ,  G01N33/56983 ,  C07K2319/33 ,  C07K2319/35 ,  C12N2770/24031 ,  C12N2770/24122 ,  C12N2770/24131 ,  C12N2770/24151 ,  G01N2333/185 ,  G01N2469/20 ,  G01N2800/26

Abstract: The disclosure relates to a polypeptide suitable for detecting antibodies against a flavivirus in an isolated biological sample having a flavivirus NS1 wing domain specific amino acid sequence, wherein no amino acid sequences from the NS1 β-ladder domain of said flavivirus are present in the polypeptide. In an embodiment, the flavivirus is selected from Zika virus (ZIKV), West-Nile virus (WNV), Dengue virus types 1-4 (DENV1-4), tick-borne encephalitis virus (TBEV), yellow fever virus (YFV) and Japanese encephalitis virus (JEV). Also disclosed is a method for producing said flaviviral NS1 wing domain specific polypeptides, a method for detecting antibodies specific for a first flavivirus species, the use of said flaviviral NS1 wing domain specific polypeptides for detecting antibodies as well as a reagent kit for detecting said flavivirus antibodies that has a flavivirus NS1 wing domain polypeptide.

公开(公告)号：US20240059746A1

公开(公告)日：2024-02-22

申请号：US18506022

申请日：2023-11-09

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Juliane Benz ,  Simon Jochum ,  Matthias Hemann

IPC: C07K14/435 ,  C07K14/005 ,  G01N33/569 ,  G01N33/58

CPC classification number: C07K14/435 ,  C07K14/005 ,  G01N33/56988 ,  G01N33/58 ,  G01N33/581 ,  C07K2319/40 ,  G01N2333/16 ,  G01N2469/20

Abstract: Fusion polypeptides are disclosed which are substrates for Kutzneria albida transglutaminase. The fusion polypeptides comprise one or more FKBP chaperone(s) and a target polypeptide. Each of these elements is separated from the neighboring element by a linker amino acid sequence. It was found that inserting glutamic acid containing transglutaminase recognition motifs into the linker amino acid chains is advantageous. Subsequent labeling reactions catalyzed by the transglutaminase surprisingly provide labeled fusion polypeptides have superior properties when compared with chemically random-labeled fusion polypeptides of similar design. Assays and kits are provided for in vitro detection of target antibodies in samples.

公开(公告)号：US20230120988A1

公开(公告)日：2023-04-20

申请号：US17907039

申请日：2021-04-22

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Frederik Baumkoetter ,  Juliane Benz ,  Markus Eckl ,  Peter Muench ,  Alexander Riedel ,  Christian Scholz

IPC: G01N33/569 ,  C07K14/005

Abstract: The present invention relates to a Corona antigen comprising a Corona nucleocapsid specific amino acid sequence, compositions, and reagent kits comprising the same and methods of producing it. Also encompassed are methods of detecting anti-Corona antibodies in samples using said Corona antigen, and methods of differential diagnosis of an immune response in a patient due to natural Corona infection or due to vaccination against Corona.

公开(公告)号：US20220220158A1

公开(公告)日：2022-07-14

申请号：US17705704

申请日：2022-03-28

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Elke Faatz ,  Alexander Riedel ,  Christian Scholz ,  Peter Muench ,  Gloria Tabares ,  Mario Gloeck ,  Silke Luebcke ,  Juliane Benz

IPC: C07K14/18 ,  C12N7/00 ,  G01N33/569 ,  C07K14/005

Abstract: The disclosure relates to a polypeptide suitable for detecting antibodies against a flavivirus in an isolated biological sample having a flavivirus NS1 wing domain specific amino acid sequence, wherein no amino acid sequences from the NS1 β-ladder domain of said flavivirus are present in the polypeptide. In an embodiment, the flavivirus is selected from Zika virus (ZIKV), West-Nile virus (WNV), Dengue virus types 1-4 (DENV1-4), tick-borne encephalitis virus (TBEV), yellow fever virus (YFV) and Japanese encephalitis virus (JEV). Also disclosed is a method for producing said flaviviral NS1 wing domain specific polypeptides, a method for detecting antibodies specific for a first flavivirus species, the use of said flaviviral NS1 wing domain specific polypeptides for detecting antibodies as well as a reagent kit for detecting said flavivirus antibodies that has a flavivirus NS1 wing domain polypeptide.

公开(公告)号：US20210349090A1

公开(公告)日：2021-11-11

申请号：US16867750

申请日：2020-05-06

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Alexander Riedel ,  Christian Scholz ,  Peter Muench ,  Juliane Benz ,  Markus Eckl ,  Frederik Baumkoetter

IPC: G01N33/569 ,  C07K14/005

Abstract: The present description relates to a Corona antigen having a Corona nucleocapsid specific amino acid sequence, compositions, and reagent kits having the same and methods of producing it. Also encompassed are methods of detecting anti-Corona antibodies in samples using the Corona antigen, and methods of differential diagnosis of an immune response in a patient due to natural Corona infection or due to vaccination against Corona.