公开(公告)号：US08980556B2

公开(公告)日：2015-03-17

申请号：US13930638

申请日：2013-06-28

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Reinhard Buettner ,  Stefanie Froehner ,  Sabine Merkelbach-Bruse ,  Jasmin Ney ,  Angelika Roesler

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  C12N9/14 ,  C12Q2600/106 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Y306/05002

Abstract: Compositions and methods for determining an increased likelihood of a response to a targeted treatment of a cancer disease including isolating genomic DNA from a patient sample, amplifying a fragment of DNA by means of PCR with a specific pair of amplification primers, determining if the amplified fragment comprises a wildtype sequence or a mutation by means of a High Resolution Melting Analysis (HRM), and correlating the presence or absence of a mutation with an increased likelihood of success of said targeted treatment. Respective primer pairs, compositions and kits are also claimed.

Abstract translation: 用于确定对癌症疾病的靶向治疗的响应的可能性增加的组合物和方法，包括从患者样品分离基因组DNA，通过用特异性的一对扩增引物扩增DNA片段，确定扩增片段 包括通过高分辨率熔解分析（HRM）的野生型序列或突变，并且将突变的存在或不存在与所述靶向治疗的成功增加的可能性相关联。 还要求各种引物对，组合物和试剂盒。