公开(公告)号：US20240294877A1

公开(公告)日：2024-09-05

申请号：US18573536

申请日：2022-06-28

Applicant: SAGA UNIVERSITY

Inventor： Koichi NAKAYAMA ,  Anna Maria NAKAMURA

IPC: C12N5/077

CPC classification number: C12N5/0655 ,  C12N2501/135 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2506/45 ,  C12N2513/00

Abstract: A method for producing a three-dimensional structure of cartilage cells, the method being characterized by comprising layering cell aggregates that have been produced by culturing a mesenchymal stem cell using a culture medium for inducing the differentiation into a cartilage cell on the 9th or 10th day after the starting of the culture.

公开(公告)号：US20240318113A1

公开(公告)日：2024-09-26

申请号：US18578809

申请日：2022-07-19

Applicant: SAGA UNIVERSITY

Inventor： Koichi NAKAYAMA ,  Anna Maria NAKAMURA

IPC: C12M1/12 ,  C12N5/00

CPC classification number: C12M25/14 ,  C12N5/0062

Abstract: A culture system that comprises: a support for stacking cell aggregates, said support being provided with needle-shaped parts that are arranged on a substrate like a kenzan (flower frog needles), in which the outermost needle-shaped parts on the substrate are arranged so that the distance from the needle-shaped parts to the outer edge of the substrate is shorter than the distance between adjacent needle-shaped parts; a culture tank for housing a culture unit in which cell aggregates are stacked on the support; a liquid culture medium container; and a plurality of liquid culture medium supply pipes that supply a liquid culture medium from the liquid culture medium container to the culture unit, wherein each of the plurality of liquid culture medium supply pipes is positioned between the needle-shaped part.