公开(公告)号：US20240077423A1

公开(公告)日：2024-03-07

申请号：US17795351

申请日：2021-03-21

Applicant: SHANDONG UNIVERSITY

Inventor： Chao GAO ,  Dan XIAO ,  Wen ZHANG ,  Cuiqing MA ,  Ping XU

IPC: G01N21/64 ,  C07K14/195 ,  C12N15/70 ,  G01N21/76

CPC classification number: G01N21/6486 ,  C07K14/195 ,  C12N15/70 ,  G01N21/763 ,  C07K2319/21

Abstract: A transcriptional regulator specifically responding to D-2-hydroxyglutarate (D-2-HG) and its application in the biological detection of D-2-HG. Wherein the transcriptional regulator is named DhdR and the nucleotide sequence is shown as SEQ ID NO: 1. The D-2-HG biosensors BD2HG-0 and BD2HG-1 are constructed using the transcriptional regulator DhdR and can detect biological samples containing D-2-HG.

公开(公告)号：US20220260496A1

公开(公告)日：2022-08-18

申请号：US17626801

申请日：2021-03-12

Applicant: SHANDONG UNIVERSITY

Inventor： Chao GAO ,  Zhaoqi KANG ,  Cuiqing MA ,  Ping XU

IPC: G01N21/64 ,  C12N15/85 ,  C12N15/70 ,  C07K14/21

Abstract: A transcriptional regulator LhgR is specifically responsive to L-2-hydroxyglutarate (L-2-HG) and an L-2-HG biosensor based on this transcriptional regulator; wherein the biosensor is a fusion protein of cyan fluorescent protein mTFP, L-2-HG specific transcriptional regulator LhgR, and yellow fluorescent protein Venus, including three types of L-2-HG biosensor LHGFR0N0C, LHGFR0N3C, and LHGFR0N7C. The application of the L-2-HG biosensor in the detection of L-2-HG-containing biological samples, real-time detection of intracellular L-2-HG concentration in bacteria and in human cells. The experiments confirmed that the biosensor can achieve high specificity, sensitivity, and accuracy in the detection of L-2-HG and can determine intracellular L-2-HG dynamics in real time, which has good application prospects.