公开(公告)号：US20240400604A1

公开(公告)日：2024-12-05

申请号：US18732149

申请日：2024-06-03

Applicant: SHANDONG UNIVERSITY

Inventor： Rong CAI ,  Can GAO ,  Mengxuan LI ,  Zhiming WANG ,  Jing TAN ,  Wenwen LI ,  Jing XU

IPC: C07H19/20 ,  C12Q1/48 ,  G01N30/02 ,  G01N30/72 ,  G01N30/88 ,  G01N33/58

Abstract: A nucleoside triphosphate photoaffinity probe, method for preparing same, and applications thereof. The nucleoside triphosphate photoaffinity probe is a novel small molecule activity probe for detecting nucleoside triphosphate-binding proteins and is based on the structure of nucleoside triphosphates (GTP and ATP) connected to a smaller photoaffinity side chain modification. This probe can effectively label nucleoside triphosphate-binding proteins in cell lysates for high-throughput proteomics analysis, identify and analyze the binding sites of the probe, and can also be used to analyze the action sites of nucleoside triphosphate competitive inhibitors, thus having significant practical application value.