公开(公告)号：US20180087022A1

公开(公告)日：2018-03-29

申请号：US15704731

申请日：2017-09-14

Applicant: SHIBUYA CORPORATION

Inventor： Masayo TAKAHASHI ,  Naoshi KOIDE ,  Takuya ONISHI ,  Motoi SASAKI ,  Masanari EJIRI

IPC: C12M1/00 ,  C12N5/00

CPC classification number: C12M47/02 ,  C12M23/38 ,  C12M23/50 ,  C12M25/02 ,  C12M37/00 ,  C12N5/0068 ,  C12N2533/54

Abstract: The present invention relates to a method for cutting a cell sheet S, which includes cutting the cell sheet S that is cultured in the inside of a container 4 which has accommodated a culture medium M therein, by a cutting device 3 that emits a laser L.The cutting method includes: removing the culture medium M from the container 4 and also sealing the container 4 by a lid portion 13 provided with a transmission part made from a material which allows the laser L to pass through, in the inside of an aseptic room 2 that has been kept in an aseptic condition; taking out the container 4 sealed by the lid portion 13, from the aseptic room 2, and moving the container to the cutting device 3 that is provided in the outside of the aseptic room 2; and in the cutting device 3, passing the laser L through the transmission part of the lid portion 13, and cutting the cell sheet S by the laser L. The method enables the cell sheet S to be cut in such a state that the aseptic condition is kept.