Abstract:
Microbial cells having a bland taste and improved odor characteristics for use in food preparations are prepared by solvent extraction. The process involves drying microbial cells after separation from a fermentation broth to a moisture content of 8 percent or less, extracting the dried cells with an aqueous alcoholic solvent containing 60 to 80 vol percent of an aliphatic alcohol having 1 to 3 carbon atoms per molecule, separating the solvent from the cells and drying the cells to yield a substantially solvent-free product. The dried product can be further treated by grinding, wetting with water and re-drying.
Abstract:
Yeasts are grown aseptically by an improved aerobic fermentation process, employing an aqueous ethanolic substrate fortified with nutrient elements, preferably under oxygen-limited conditions. Fermentation is effected in a continuous manner in a zone maintained under super-atmospheric pressure. Micro-nutrients are added to the substrate separately from macro-nutrients as a sterile aqueous solution, preferably containing iron as ferric citrate. A preferred yeast is Candida utilis.
Abstract:
Protein-containing microbial cells are treated to remove undesirable flavor and odor substances by a process involving heat-killing the cells, drying the cells, extracting the dried cells with an aqueous alcoholic solution containing 60 to 80 vol. percent of an alcohol such as ethanol, filtering to separate the extracted microbial cells from the aqueous alcoholic solution and drying the extracted microbial cells to provide substantially solvent-free microbial cells.