公开(公告)号：US20150148486A1

公开(公告)日：2015-05-28

申请号：US14610681

申请日：2015-01-30

Applicant: SUMITOMO BAKELITE COMPANY, LTD.

Inventor： Sohei FUNAOKA ,  Midori ABE

IPC: G01N33/531

CPC classification number: G01N33/531 ,  B05D3/00 ,  C07K17/00 ,  C08F290/046 ,  C09D133/10 ,  C12N15/1006 ,  C12Q2565/50 ,  G01N33/54393

Abstract: An object of the present invention is to provide a method of immobilizing the biologically active substance which has an excellent capability of immobilizing a target biologically active substance, and exhibits low nonspecific adsorption of the biologically active substance to provide a high S/N ratio, without using a functional group for fixing the biologically active substance and without having a process of inactivating the functional group for fixing the biologically active substance after immobilizing the biologically active substance. The above object is achieved by a method of immobilizing a biologically active substance comprising the step of: bringing a solution into contact with a compound-side surface of an immobilizing substrate to immobilize the biologically active substance on a surface of the immobilizing substrate, the solution being prepared by dissolving the biologically active substance in a phosphate buffer having a phosphate concentration of 0.1 M or more, and the immobilizing substrate comprising a substrate and a compound containing a hydrophilic group inhibiting nonspecific adsorption on a surface of the substrate.

Abstract translation: 本发明的目的是提供一种固定化生物活性物质固定化能力优异的生物活性物质的方法，并且生物活性物质的非特异性吸附低，提供高的S / N比，而没有 使用固定生物活性物质的官能团，并且在固定生物活性物质之后没有使用于固定生物活性物质的官能团失活的方法。 上述目的是通过固定生物活性物质的方法实现的，包括以下步骤：使溶液与固定基质的化合物侧表面接触，将生物活性物质固定在固定基材的表面上，溶液 通过将生物活性物质溶解在磷酸盐浓度为0.1M以上的磷酸盐缓冲液中，将含有底物的固定化基质和含有抑制非特异性吸附的亲水基团的化合物在基材的表面上进行制备。