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公开(公告)号:US20220195479A1
公开(公告)日:2022-06-23
申请号:US17600033
申请日:2020-04-01
Applicant: Spiber Inc.
Inventor: Masato KOBAYASHI , Kenji KURACHI , Eiji MORI , Takanobu NODA
Abstract: The present invention relates to a method for producing a recombinant protein, the method including: a first step of culturing a recombinant cell expressing a recombinant protein under control of an inducible promoter in a medium containing galactose and at least two selected from the group consisting of mannitol, raffinose, and melibiose; and a second step of further culturing the recombinant cell under a condition that expression from the inducible promoter is induced after the first step.