公开(公告)号：US20190225646A1

公开(公告)日：2019-07-25

申请号：US16339119

申请日：2017-10-03

Applicant: Spiber Inc.

Inventor： Toshimasa Homma

IPC: C07K1/30 ,  C07K14/78 ,  D01F6/68 ,  D01D1/02 ,  D01D5/06

Abstract: Disclosed is a method for purifying a recombinant protein of interest from a recombinant cell expressing intracellularly the recombinant protein as an insoluble matter, comprising treating the recombinant cell under conditions where a protein derived from a host cell dissolves in a first aprotic polar solvent to which an inorganic salt is added or not but the recombinant protein does not dissolve, removing a first soluble fraction, and then treating the resulting first insoluble fraction under conditions where the recombinant protein dissolves in a second aprotic polar solvent to which an inorganic salt is added.