公开(公告)号：US10899792B2

公开(公告)日：2021-01-26

申请号：US16324280

申请日：2017-08-10

Applicant: Spiber Inc.

Inventor： Toshiaki Osawa ,  Yuya Sato ,  Keisuke Morita

IPC: C07K1/34 ,  C07K1/30 ,  C12P21/02 ,  C12P21/00 ,  C07K1/14

Abstract: An object of the present invention is to provide a method for efficiently separating insoluble bodies of a recombinant protein from a recombinant cell expressing a target recombinant protein as insoluble bodies in the cell. The present invention provides a method for producing a recombinant protein aggregate by separating insoluble bodies of a recombinant protein from a recombinant cell expressing the recombinant protein as insoluble bodies in the cell, including disrupting the recombinant cell, aggregating the insoluble bodies of the recombinant protein, and separating the resulting aggregate.

公开(公告)号：US20190177363A1

公开(公告)日：2019-06-13

申请号：US16324280

申请日：2017-08-10

Applicant: Spiber Inc.

Inventor： Toshiaki Osawa ,  Yuya Sato ,  Keisuke Morita

IPC: C07K1/34 ,  C07K1/30 ,  C12P21/02

Abstract: An object of the present invention is to provide a method for efficiently separating insoluble bodies of a recombinant protein from a recombinant cell expressing a target recombinant protein as insoluble bodies in the cell. The present invention provides a method for producing a recombinant protein aggregate by separating insoluble bodies of a recombinant protein from a recombinant cell expressing the recombinant protein as insoluble bodies in the cell, including disrupting the recombinant cell, aggregating the insoluble bodies of the recombinant protein, and separating the resulting aggregate.