公开(公告)号：US11578265B2

公开(公告)日：2023-02-14

申请号：US16319436

申请日：2017-07-21

Applicant: THE HONG KONG UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Benzhong Tang ,  Xiujuan Shi ,  Yee Yung Yu ,  Tsz Kin Kwok ,  Zheng Zhao ,  Pengfei Zhang ,  Huifang Su

IPC: C09K11/06 ,  C07D221/14 ,  C07D213/57 ,  C07D401/12 ,  C07D285/14 ,  A61N5/06 ,  G01N33/533 ,  G01N33/58 ,  C07C211/43

Abstract: The present subject matter is directed to a luminogen exhibiting aggregation induced emission, wherein T1, T2, and T3 comprise one or more polyynes as a conjugated bridge. The present subject matter is also directed to an AIEgen comprising a hydrophilic pyridium group as a strong electron-withdrawing group; a piperazine group as an electron-donating group; and a α-Cyanostilbene; wherein the AIEgen exhibits aggregation induced emission. The present subject matter is directed to a method of synthesizing an AIEgen and is further directed to a method of labeling comprising incubating a subject having cells with a conjugate formed by conjugating an AIEgen with an antibody; and selectively labeling desired cells by turn-on imaging, wherein labeling occurs when the desired cells are selectively stained by fluorescent emission of the AIEgen upon degradation of the antibody after cellular internalization of the conjugate through endocytosis.