公开(公告)号：US20240255527A1

公开(公告)日：2024-08-01

申请号：US18563745

申请日：2022-05-24

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Aydogan Ozcan ,  Hyou-Arm Joung ,  Yi Luo

IPC: G01N33/68 ,  G01N15/075 ,  G01N33/543 ,  G06T7/00 ,  G06V10/762 ,  G06V10/82 ,  G06V20/69

CPC classification number: G01N33/6893 ,  G01N15/075 ,  G01N33/54388 ,  G06T7/0012 ,  G06V10/762 ,  G06V10/82 ,  G06V20/698 ,  G01N2333/4737 ,  G01N2800/7095 ,  G06T2207/10056 ,  G06T2207/20084 ,  G06T2207/30004 ,  G06T2207/30204

Abstract: A quantitative particle agglutination assay device is disclosed that combines portable lens-free microscopy and deep learning for rapidly measuring the concentration of a target analyte. As one example of a target analyte, the assay device was used to test for high-sensitivity C-reactive protein (hs-CRP) using human serum samples. A dual-channel capillary lateral flow device is designed to host the agglutination reaction using a small volume of serum. A portable lens-free microscope records time-lapsed inline holograms of the lateral flow device, monitoring the agglutination process over several minutes. These captured holograms are processed, and at each frame the number and area of the particle clusters are automatically extracted and fed into shallow neural networks to predict the CRP concentration. The system can be used to successfully differentiate very high CRP concentrations (e.g., >10-500 μg/mL) from the hs-CRP range.

公开(公告)号：US20210382052A1

公开(公告)日：2021-12-09

申请号：US17285906

申请日：2019-10-18

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Hyou-Arm Joung ,  Zachary S. Ballard ,  Omai Garner ,  Dino Di Carlo ,  Aydogan Ozcan

IPC: G01N33/569 ,  B01L3/00

Abstract: A multiplexed vertical flow serodiagnostic testing device for diseases such as Lyme disease includes one or more multi-piece cassettes that include vertical stacks of functionalized porous layers therein. A bottom piece of the cassette includes a sensing membrane with a plurality of spatially multiplexed immunoreaction spots or locations. Top pieces are used to deliver sample and/or buffer solutions along with antibody-conjugated nanoparticles for binding with the immunoreaction spots or locations. A colorimetric signal is generated by the nanoparticles captured on the sensing membrane containing disease-specific antigens. The sensing membrane is imaged by a cost-effective portable reader device. The images captured by the reader device are subject to image processing and analysis to generate positive (+) or negative (−) indication for the sample. A concentration of one or more biomarkers may also be generated. The testing device is rapid, simple, inexpensive, and allows for simultaneous measurement of multiple antibodies and/or antigens making it an ideal point-of-care platform for disease diagnosis.

公开(公告)号：US20220299525A1

公开(公告)日：2022-09-22

申请号：US17612575

申请日：2020-05-22

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Aydogan Ozcan ,  Hyou-Arm Joung ,  Zachary S. Ballard ,  Omai Garner ,  Dino Di Carlo ,  Artem Goncharov

IPC: G01N33/68 ,  G01N33/543 ,  G06T7/00

Abstract: A system for detecting the presence of and/or quantifying the amount or concentration of one or more analytes in a sample includes a flow assay cartridge having a multiplexed sensing membrane that has immunoreaction or biological reaction spots of varying conditions spatially arranged across the surface of the membrane defining an optimized spot map. A reader device is provided that uses a camera to image the multiplexed sensing membrane. Image processing software obtains normalized pixel intensity values of the plurality of immunoreaction or biological reaction spots and which are used as inputs to one or more trained neural networks configured to generate one or more outputs that: (i) quantify the amount or concentration of the one or more analytes in the sample; and/or (ii) indicate the presence of the one or more analytes in the sample; and/or (ii) determines a diagnostic decision or classification of the sample.

公开(公告)号：US12013395B2

公开(公告)日：2024-06-18

申请号：US17285906

申请日：2019-10-18

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Hyou-Arm Joung ,  Zachary S. Ballard ,  Omai Garner ,  Dino Di Carlo ,  Aydogan Ozcan

IPC: G01N33/569 ,  B01L3/00 ,  B82Y5/00 ,  B82Y30/00 ,  B82Y40/00

CPC classification number: G01N33/56911 ,  B01L3/502715 ,  B01L2300/0819 ,  B01L2300/126 ,  B01L2400/0406 ,  B82Y5/00 ,  B82Y30/00 ,  B82Y40/00 ,  G01N2333/20

Abstract: A multiplexed vertical flow serodiagnostic testing device for diseases such as Lyme disease includes one or more multi-piece cassettes that include vertical stacks of functionalized porous layers therein. A bottom piece of the cassette includes a sensing membrane with a plurality of spatially multiplexed immunoreaction spots or locations. Top pieces are used to deliver sample and/or buffer solutions along with antibody-conjugated nanoparticles for binding with the immunoreaction spots or locations. A colorimetric signal is generated by the nanoparticles captured on the sensing membrane containing disease-specific antigens. The sensing membrane is imaged by a cost-effective portable reader device. The images captured by the reader device are subject to image processing and analysis to generate positive (+) or negative (−) indication for the sample. A concentration of one or more biomarkers may also be generated. The testing device is rapid, simple, inexpensive, and allows for simultaneous measurement of multiple antibodies and/or antigens making it an ideal point-of-care platform for disease diagnosis.