公开(公告)号：US20190137932A1

公开(公告)日：2019-05-09

申请号：US16302087

申请日：2017-05-24

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Aydogan Ozcan ,  Yibo Zhang ,  Seung Yoon Lee ,  John D. Fitzgerald

IPC: G03H1/04 ,  G03H1/00 ,  G03H1/08 ,  H04N5/232 ,  G06T5/50

Abstract: A method of imaging a sample having birefringent crystals (or other materials) using a lens-free polarized microscopy device includes illuminating the sample contained on a sample holder with circularly polarized partially coherent or coherent light and capturing lower resolution holographic images of the birefringent crystals with an image sensor. A polarization analyzer unit made from a λ/4 retarder and a linear polarizer is positioned between the sample holder and the image sensor. The lower resolution holographic images are obtained with the polarization analyzer unit in two different orientations (e.g. ˜90° orientations). Phase-retrieved, higher resolution images of the birefringent crystals at the different orientations are obtained using the lower resolution holographic images. A differential image is generated from the respective phase-retrieved, higher resolution images. An object support mask is applied to identify the birefringent crystals which can then be pseudo-colored.