公开(公告)号：US11492652B2

公开(公告)日：2022-11-08

申请号：US16165074

申请日：2018-10-19

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Shiro Kitano ,  Kei Tsukamoto ,  Shinji Irie

IPC: C12Q1/02

Abstract: A method of assessing a possibility of cancerization including culturing a cell structure including normal cells and having a vascular network structure in a presence of a biological specimen from a subject, and assessing a possibility of cancerization in the subject based on a state of vessels in the cell structure after the culturing. The biological specimen is a body fluid specimen from the subject, a cell extract of cells from the subject, or a culture supernatant of cells from the subject, and the possibility of cancerization is assessed as high in the subject when a number of cells forming the vessels in the cell structure is larger than a number of cells cultured in an absence of the body fluid specimen, or when the vascular network structure in the cell structure extends.

公开(公告)号：US11254917B2

公开(公告)日：2022-02-22

申请号：US16104533

申请日：2018-08-17

Applicant: TOPPAN PRINTING CO., LTD. ,  OSAKA UNIVERSITY

Inventor： Kei Tsukamoto ,  Shinji Irie ,  Michiya Matsusaki

IPC: C12N5/07 ,  C12N5/071 ,  C12N5/09 ,  C12M3/00

Abstract: A method of promoting spheroid formation, including: a preparation step of preparing a mixture obtained by mixing a cell sample with a promoter; and a culture step of culturing, inside a spheroid formation-culture vessel, the mixture obtained in the preparation step, in which the promoter is a polymer in which one or more selected from the group consisting of D-glucosamine, D-galactosamine, D-glucuronic acid, L-iduronic acid, and D-galactose are polymerized.

公开(公告)号：US11479764B2

公开(公告)日：2022-10-25

申请号：US16951456

申请日：2020-11-18

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Hiroki Nagase ,  Kei Tsukamoto ,  Shiro Kitano

IPC: C12N15/10 ,  C12N15/09 ,  C07H1/06 ,  C07H21/00 ,  C08G69/10 ,  C08G73/06 ,  G01N1/40

Abstract: A method of separating a target double-stranded nucleic acid molecule from a sample including the target double-stranded nucleic acid molecule and a non-target double-stranded nucleic acid molecule, including (1) mixing the sample, a pyrrole-imidazole-containing polyamide (first PI polyamide) modified with a first linker molecule and capable of specifically binding to a sequence of the target double-stranded nucleic acid molecule, and a carrier a modified with a first ligand capable of specifically binding and/or adsorbing to the first linker molecule such that a mixed solution is produced, (2) forming a complex A by binding the carrier a to the first PI polyamide with which the target double-stranded nucleic acid molecule is bound in the mixed solution, and (3) separating the complex A from the mixed solution.

公开(公告)号：US11249070B2

公开(公告)日：2022-02-15

申请号：US16164989

申请日：2018-10-19

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Shiro Kitano ,  Kei Tsukamoto ,  Shinji Irie

IPC: G01N33/50 ,  C12N5/09 ,  C12N5/071

Abstract: A method of assessing an anti-cancer drug including culturing a cell structure including cancer cells and stromal cells in a presence of at least one anti-cancer drug, and assessing an anti-cancer effect of the at least one anti-cancer drug based on a number of viable cancer cells in the cell structure after the culturing.

公开(公告)号：US11221326B2

公开(公告)日：2022-01-11

申请号：US16164914

申请日：2018-10-19

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Shiro Kitano ,  Kei Tsukamoto ,  Shinji Irie

IPC: C12P1/00 ,  G01N33/50

Abstract: A method of producing drug resistant cells including contacting exosomes from a living organism exhibiting drug resistance with cells exhibiting no drug resistance such that drug resistant cells are formed, and culturing the drug resistant cells.

公开(公告)号：US10858646B2

公开(公告)日：2020-12-08

申请号：US15948124

申请日：2018-04-09

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Hiroki Nagase ,  Kei Tsukamoto ,  Shiro Kitano

IPC: C12N15/10 ,  C08G69/10 ,  C08G73/06 ,  G01N1/40 ,  C12N15/09 ,  C07H1/06 ,  C07H21/00

Abstract: A method of separating a target double-stranded nucleic acid molecule from a sample including the target double-stranded nucleic acid molecule and a non-target double-stranded nucleic acid molecule, including (1) mixing the sample, a pyrrole-imidazole-containing polyamide (first PI polyamide) modified with a first linker molecule and capable of specifically binding to a sequence of the target double-stranded nucleic acid molecule, and a carrier a modified with a first ligand capable of specifically binding and/or adsorbing to the first linker molecule such that a mixed solution is produced, (2) forming a complex A by binding the carrier a to the first PI polyamide with which the target double-stranded nucleic acid molecule is bound in the mixed solution, and (3) separating the complex A from the mixed solution.

公开(公告)号：US10323241B2

公开(公告)日：2019-06-18

申请号：US15292880

申请日：2016-10-13

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Kei Tsukamoto

IPC: C07H1/08 ,  C12Q1/68 ,  C12N15/10 ,  C12Q1/6806

Abstract: A method of recovering short-chain nucleic acids having a length of 1000 bases or less from a whole blood sample, which includes bringing a first mixture including a whole blood sample into contact with a first nucleic acid adsorbent to adsorb nucleic acids derived from the whole blood sample, removing the first nucleic acid adsorbent from being in contact with the first mixture to recover liquid components of the first mixture, bringing a second mixture including the recovered liquid components into contact with a second nucleic acid adsorbent to adsorb short-chain nucleic acids having a length of 1000 bases or less from the whole blood sample, separating the second nucleic acid adsorbent from being in contact with the second mixture, and eluting the short-chain nucleic acids absorbed in the second nucleic acid adsorbent and recovering the short-chain nucleic acids from the second nucleic acid adsorbent.

公开(公告)号：US20190049431A1

公开(公告)日：2019-02-14

申请号：US16164914

申请日：2018-10-19

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Shiro KITANO ,  Kei Tsukamoto ,  Shinji Irie

IPC: G01N33/50

Abstract: A method of producing drug resistant cells including contacting exosomes from a living organism exhibiting drug resistance with cells exhibiting no drug resistance such that drug resistant cells are formed, and culturing the drug resistant cells.

公开(公告)号：US20190048387A1

公开(公告)日：2019-02-14

申请号：US16165074

申请日：2018-10-19

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Shiro Kitano ,  Kei Tsukamoto ,  Shinji Irie

IPC: C12Q1/02

Abstract: A method of assessing a possibility of cancerization including culturing a cell structure including normal cells and having a vascular network structure in a presence of a biological specimen from a subject, and assessing a possibility of cancerization in the subject based on a state of vessels in the cell structure after the culturing. The biological specimen is a body fluid specimen from the subject, a cell extract of cells from the subject, or a culture supernatant of cells from the subject, and the possibility of cancerization is assessed as high in the subject when a number of cells forming the vessels in the cell structure is larger than a number of cells cultured in an absence of the body fluid specimen, or when the vascular network structure in the cell structure extends.