ALKALINE PHOSPHATASE COMPOSITION, METHOD OF PRODUCING DEPHOSPHORYLATED NUCLEIC ACID AND METHOD OF PRODUCING LABELED NUCLEIC ACID

    公开(公告)号:US20210395708A1

    公开(公告)日:2021-12-23

    申请号:US17279686

    申请日:2019-09-25

    Abstract: A composition contains an alkaline phosphatase and first to sixth peptide fragments, wherein content ratios of the first to sixth peptide fragments to the alkaline phosphatase satisfy formulas (1) to (6), respectively: (X1/Y)×100≤0.6000 (1); (X2/Y)×100≤0.1800 (2); (X3/Y)×100≤0.2000 (3); (X4/Y)×100≤0.8000 (4); (X5/Y)×100≤1.6000 (5); and (X6/Y)×100≤0.3500 (6), wherein X1 to X6 represent peak area values of the first to sixth peptide fragments calculated by an automatic integration method from an extracted ion chromatogram obtained by an LC-MS/MS analysis of the composition, respectively, and Y represents a peak area value of the alkaline phosphatase calculated by an automatic integration method from a chromatogram obtained by an LC-UV analysis of the composition.

    ALKALINE PHOSPHATASE COMPOSITION, METHOD OF PRODUCING DEPHOSPHORYLATED NUCLEIC ACID AND METHOD OF PRODUCING LABELED NUCLEIC ACID

    公开(公告)号:US20210332337A1

    公开(公告)日:2021-10-28

    申请号:US17279800

    申请日:2019-09-25

    Abstract: A composition contains an alkaline phosphatase; and a peptide fragment group (A) composed of two or more peptide fragments, wherein each of the two or more peptide fragments consists of 5 to 50 consecutive amino acid residues selected from positions 501 to 578 of the amino acid sequence set forth in SEQ ID NO: 5, wherein a content ratio of the peptide fragment group (A) to the alkaline phosphatase satisfies formula (A): (XA/Y)×100≤4.4000 (A), wherein XA represents a peak area value of the peptide fragment group (A) calculated by an automatic integration method from an extracted ion chromatogram obtained by an LC-MS/MS analysis of the composition, and Y represents a peak area value of the alkaline phosphatase calculated by an automatic integration method from a chromatogram obtained by an LC-UV analysis of the composition.

    Method for producing α-hydromuconic acid

    公开(公告)号:US11124811B2

    公开(公告)日:2021-09-21

    申请号:US15580585

    申请日:2016-06-09

    Abstract: A method of producing α-hydromuconic acid using a metabolic pathway of a microorganism is disclosed. The method of producing α-hydromuconic acid includes the step of culturing at least one type of microorganism having a capacity to produce α-hydromuconic acid, selected from the group consisting of microorganisms belonging to the genus Escherichia, microorganisms belonging to the genus Pseudomonas, microorganisms belonging to the genus Hafnia, microorganisms belonging to the genus Bacillus, microorganisms belonging to the genus Cupriavidus, microorganisms belonging to the genus Acinetobacter, microorganisms belonging to the genus Alcaligenes, microorganisms belonging to the genus Delftia, and microorganisms belonging to the genus Shimwellia.

    Method for producing 3-oxoadipic acid

    公开(公告)号:US10858678B2

    公开(公告)日:2020-12-08

    申请号:US16060182

    申请日:2016-12-09

    Abstract: A method of producing 3-oxoadipic acid from an aliphatic compound easily utilizable by a microorganism, such as a saccharide, by utilization of a metabolic pathway of the microorganism is disclosed. The method of producing 3-oxoadipic acid includes the step of culturing at least one type of microorganism having a capacity to produce 3-oxoadipic acid, selected from the group consisting of, for example, microorganisms belonging to the genus Serratia, microorganisms belonging to the genus Corynebacterium, microorganisms belonging to the genus Hafnia, microorganisms belonging to the genus Bacillus, microorganisms belonging to the genus Escherichia, microorganisms belonging to the genus Pseudomonas, microorganisms belonging to the genus Acinetobacter, microorganisms belonging to the genus Alcaligenes, microorganisms belonging to the genus Shimwellia, microorganisms belonging to the genus Planomicrobium, microorganisms belonging to the genus Nocardioides, microorganisms belonging to the genus Yarrowia, microorganisms belonging to the genus Cupriavidus, microorganisms belonging to the genus Rhodosporidium, microorganisms belonging to the genus Streptomyces, and microorganisms belonging to the genus Microbacterium.

    PROCESS FOR PRODUCING 2,3-BUTANEDIOL
    8.
    发明申请
    PROCESS FOR PRODUCING 2,3-BUTANEDIOL 审中-公开
    生产2,3-丁二醇的方法

    公开(公告)号:US20140238841A1

    公开(公告)日:2014-08-28

    申请号:US14350366

    申请日:2012-10-12

    Abstract: A method of producing 2,3-butanediol includes subjecting a 2,3-butanediol culture liquid produced by microbial fermentation to nanofiltration membrane treatment and ion-exchange treatment (Step A), and then adding an alkaline substance and performing distillation (Step B).

    Abstract translation: 制备2,3-丁二醇的方法包括将通过微生物发酵生产的2,3-丁二醇培养液进行纳滤膜处理和离子交换处理(步骤A),然后加入碱性物质进行蒸馏(步骤B) 。

Patent Agency Ranking