公开(公告)号：US20210236622A1

公开(公告)日：2021-08-05

申请号：US17048714

申请日：2019-04-18

Applicant: Texas Tech University System

Inventor： Mingtao Zeng ,  Ke Wen

IPC: A61K39/145 ,  A61K39/39 ,  C12N7/00 ,  C12N15/113

Abstract: The present invention includes a live, self-attenuated therapeutic vaccine, virus and methods of making and using the same, comprising: an isolated virus comprising a viral genome that expresses one or more viral antigens; and an artificial microRNA 30 (amiR-30) expression cassette inserted into a viral neuraminidase (NA) or a viral non-structural (NS) gene segment that expresses an amiR-30 that specifically inhibits the expression of a host gene essential for influenza virus replication in host cells.