公开(公告)号：US20180094300A1

公开(公告)日：2018-04-05

申请号：US15563838

申请日：2016-03-30

Applicant: Texas Tech University System

Inventor： Marie Bugarel ,  Kendra Nightingale ,  Guy Loneragan ,  Mindy M. Brashears

IPC: C12Q1/689 ,  C12Q1/6806 ,  C12Q1/686

CPC classification number: C12Q1/689 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q2600/158 ,  C12Q2600/16 ,  Y02A50/451

Abstract: The present invention provides a set of oligonucleotides to screen for the presence of targeted Salmonella serotypes in enrichment or to characterize presumptive colonies. The set of oligonucleotides includes at least one set of primers and probe for the detection of Salmonella serotype selected from Newport, Heidelberg, Infantis, and Hadar and to discriminate between. These new markers can be used after the initial screening assay described herein as a discrimination assay to differentiate S. Heidelberg from S. Infantis and S. Hadar from S. Newport.

公开(公告)号：US20170268044A1

公开(公告)日：2017-09-21

申请号：US15129108

申请日：2015-03-26

Applicant: Texas Tech University System

Inventor： Guy Loneragan ,  Mindy M. Brashears ,  Kendra Nightingale ,  Marie Bugarel

IPC: C12Q1/68 ,  G01N33/02

CPC classification number: C12Q1/689 ,  C12Q2600/16 ,  G01N33/02 ,  Y02A50/451

Abstract: The present invention provides a set of oligonucleotides to screen for the presence of targeted Salmonella serotypes in an enrichment or to characterize presumptive colonies. The set of oligonucleotides includes at least one set of primers and probe for the detection of Salmonella serotype selected from typhimurium, enteritidis, newport, heidelberg, infantis, virchow and Hadar. The set of oligonucleotides may include up to 5 different primer sets and the corresponding probes.

公开(公告)号：US10323286B2

公开(公告)日：2019-06-18

申请号：US15563838

申请日：2016-03-30

Applicant: Texas Tech University System

Inventor： Marie Bugarel ,  Kendra Nightingale ,  Guy Loneragan ,  Mindy M. Brashears

IPC: C12Q1/68 ,  C12Q1/689 ,  C12Q1/6806 ,  C12Q1/686

Abstract: The present invention provides a set of oligonucleotides to screen for the presence of targeted Salmonella serotypes in enrichment or to characterize presumptive colonies. The set of oligonucleotides includes at least one set of primers and probe for the detection of Salmonella serotype selected from Newport, Heidelberg, Infantis, and Hadar and to discriminate between. These new markers can be used after the initial screening assay described herein as a discrimination assay to differentiate S. Heidelberg from S. Infantis and S. Hadar from S. Newport.

公开(公告)号：US10287638B2

公开(公告)日：2019-05-14

申请号：US15129108

申请日：2015-03-26

Applicant: Texas Tech University System

Inventor： Guy Loneragan ,  Mindy M. Brashears ,  Kendra Nightingale ,  Marie Bugarel

IPC: C07H21/04 ,  C12Q1/689 ,  G01N33/02

Abstract: The present invention provides a set of oligonucleotides to screen for the presence of targeted Salmonella serotypes in an enrichment or to characterize presumptive colonies. The set of oligonucleotides includes at least one set of primers and probe for the detection of Salmonella serotype selected from typhimurium, enteritidis, newport, heidelberg, infantis, virchow and Hadar. The set of oligonucleotides may include up to 5 different primer sets and the corresponding probes.