公开(公告)号：US20020103191A1

公开(公告)日：2002-08-01

申请号：US10047301

申请日：2002-01-14

Applicant: Board of Regents, The University of Texas System

Inventor： Frederick F. Becker ,  Bimal K. Banik

IPC: C07D279/12 ,  C07D265/30 ,  C07D211/06 ,  A61K031/535 ,  A61K031/495 ,  A61K031/135

CPC classification number: C07D295/185 ,  A61K31/4453 ,  A61K31/495

Abstract: Dibenzofluorene derivatives having a formula selected from the group consisting of 1 and salts thereof have antitumor activity. At least one of R1-R13 in formula (I) or R1-R12 in formula (II) is nullR14Z. R14 is a substituted or unsubstituted amino or amido group having from 1-12 carbon atoms, and Z is a substituted or unsubstituted heterocyclic group having from 1-12 carbon atoms. The remainder of R1-R13 in formula (I) or R1-R12 in formula (II) are independently selected from the group consisting of hydrogen, hydroxyl, halogen, nitro, substituted or unsubstituted amino or amido groups having from 1-12 carbon atoms, and alkyl groups having 1-12 carbon atoms.

Abstract translation: 具有选自其盐及其盐的式的二苯并芴衍生物具有抗肿瘤活性。 式（I）中的R 1 -R 13或式（II）中的R 1 -R 12中的至少一个为-R 14 Z。 R14是具有1-12个碳原子的取代或未取代的氨基或酰胺基，Z是具有1-12个碳原子的取代或未取代的杂环基。 式（I）中的R 1 -R 13或式（II）中的R 1 -R 12的其余部分独立地选自氢，羟基，卤素，硝基，取代或未取代的具有1-12个碳原子的氨基或酰胺基 ，和具有1-12个碳原子的烷基。

公开(公告)号：US20020036139A1

公开(公告)日：2002-03-28

申请号：US09902933

申请日：2001-07-10

Applicant: Board of Regents, The University of Texas System

Inventor： Frederick F. Becker ,  Peter Gascoyne ,  Xiaobo Wang ,  Jody Valentine Vykoukal ,  Giovanni De Gasperis

IPC: G01N027/26

CPC classification number: G01N27/44773 ,  B01F13/0076 ,  B01F2215/0404 ,  B01L3/502715 ,  B01L3/502761 ,  B01L3/502792 ,  B01L2200/143 ,  B01L2300/089 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0454 ,  B03C5/005 ,  B03C5/024 ,  B03C5/026 ,  B03C5/028 ,  G01N27/447

Abstract: A method and apparatus for microfluidic processing by programmably manipulating a packet. A material is introduced onto a reaction surface and compartmentalized to form a packet. A position of the packet is sensed with a position sensor. A programmable manipulation force is applied to the packet at the position. The programmable manipulation force is adjustable according to packet position by a controller. The packet is programmably moved according to the programmable manipulation force along arbitrarily chosen paths.

Abstract translation: 用于通过可编程地操纵分组的微流体处理的方法和装置。 将材料引入反应表面并分隔成形成包装。 用位置传感器检测分组的位置。 可编程操纵力被施加到该位置处的分组。 可编程操纵力可根据控制器的分组位置进行调节。 根据可编程操纵力沿着任意选择的路径可编程地移动分组。

公开(公告)号：US20040178068A1

公开(公告)日：2004-09-16

申请号：US10743698

申请日：2003-12-22

Applicant: The University of Texas System

Inventor： Peter R.C. Gascoyne ,  Jody V. Vykoukal ,  Chandra Das ,  Frederick F. Becker

IPC: G01N027/26

CPC classification number: G01N30/0005 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/24 ,  G01N1/2813 ,  G01N2030/0065 ,  G01N2030/007

Abstract: Methods and apparatus for preparing a smear for cytopathology or other analysis. In a representative embodiment, cells of a sample are subjected to a dielectrophoretic force to segregate the cells into two or more zones of a surface. The particles are attached to the surface, thereby defining a nullsegregated smear.null The segregated smear is then fixed and stained for cytopathology analysis.

Abstract translation: 用于细胞病理学或其他分析的涂片的制备方法和装置。 在代表性的实施方案中，样品的细胞经受介电电泳力以将细胞分离成表面的两个或更多个区域。 颗粒附着在表面上，从而定义了“分离的涂片”。 然后将分离的涂片固定并染色用于细胞病理学分析。

公开(公告)号：US20030173223A1

公开(公告)日：2003-09-18

申请号：US10336611

申请日：2003-01-03

Applicant: Board of Regents,The University of Texas System

Inventor： Peter R.C. Gascoyne ,  Jody Vykoukal ,  Frederick F. Becker

IPC: G01N027/403 ,  G01N027/453

CPC classification number: B03C5/028

Abstract: Methods and apparatuses for providing wall-less virtual channels. The wall-less channels may be regions such as stripes or other patterns that are defined by polar surface coatings. These wall-less channel may be used with polar solvents and defined by polar surface patterning of narrowly separated top and bottom walls of a chamber filled elsewhere by a non-polar partitioning medium. This provides a simple and easy-to-fabricate interface between the micro and macro worlds in which microfluidic processes are separated from the macro world fluid flow by a narrow veil of immiscible fluid across which an exchange of droplets can be controlled electrically.

Abstract translation: 用于提供无墙虚拟通道的方法和装置。 无壁通道可以是由极性表面涂层限定的区域，例如条纹或其它图案。 这些无壁通道可以与极性溶剂一起使用，并且通过由非极性分配介质在其他地方填充的室的狭窄分离的顶壁和底壁的极性表面图案化来限定。 这提供了在微观和宏观世界之间的简单和容易制造的界面，其中微流体过程通过狭窄的不混溶流体面与宏观世界流体流分离，跨液体流动可以控制液滴的交换。

公开(公告)号：US20030171325A1

公开(公告)日：2003-09-11

申请号：US10336610

申请日：2003-01-03

Applicant: Board of Regents, The University of Texas System

Inventor： Peter R.C. Gascoyne ,  Daynene Vykoukal ,  Frederick F. Becker

IPC: A61K031/70 ,  A01N043/04

CPC classification number: B82Y30/00 ,  C12N15/10 ,  C12P19/34

Abstract: Methods and apparatuses for solid-phase oligonucleotide synthesis and forming long polynucleotides. One exemplary method includes synthesizing a sense oligonucleotide; synthesizing an antisense oligonucleotide; annealing the sense and antisense oligonucleotides to form double stranded DNA (dsDNA); capping the ends of the dsDNA; cleaving the dsDNA wherein cleavage occurs at or near a Watson-Crick base pair mismatch; and digesting uncapped dsDNA. Another exemplary method includes synthesizing a first proofread double stranded DNA (dsDNA); synthesizing a second proofread dsDNA; and ligating the first proofread DNA with the second proofread DNA to form a long polynucleotide.

Abstract translation: 固相寡核苷酸合成和形成长多核苷酸的方法和装置。 一种示例性方法包括合成有义寡核苷酸; 合成反义寡核苷酸; 对正义和反义寡核苷酸进行退火以形成双链DNA（dsDNA）; 盖上dsDNA的末端; 切割双链DNA，其中切割发生在沃森 - 克里克碱基对不匹配或接近; 并消化未加盖的dsDNA。 另一个示例性方法包括合成第一校对双链DNA（dsDNA）; 合成第二个校对dsDNA; 并将第一校样DNA与第二校样DNA连接以形成长多核苷酸。