公开(公告)号：US20180258457A1

公开(公告)日：2018-09-13

申请号：US15959498

申请日：2018-04-23

Applicant: UCB BIOPHARMA SPRL

Inventor： PHILIP JONATHAN BASSETT ,  DAVID PAUL HUMPHREYS ,  PARESHKUMAR MANJIBHAI PATEL

IPC: C12P21/00 ,  C12N9/52 ,  C12P21/02 ,  C07K16/28 ,  C12N15/70

Abstract: The present disclosure relates to a recombinant gram-negative bacterial cell comprising: a) a mutant spr gene encoding a spr protein having a mutation at one or more amino acids selected from D133, H145, H157, N31, R62, I70, Q73, C94, S95, V98, Q99, R100, L108, Y115, V135, L136, G140, R144 and G147 and b) a gene capable of expressing or overexpressing one or more proteins capable of facilitating protein folding, such as FkpA, Skp, SurA, PPiA and PPiD, wherein the cell has reduced Tsp protein activity compared to a wild-type cell, methods employing the cells, use of the cells in the expression of proteins in particular antibodies, such as anti FcRn antibodies and proteins made by the methods described herein.

公开(公告)号：US20200071387A1

公开(公告)日：2020-03-05

申请号：US16674003

申请日：2019-11-05

Applicant: UCB BIOPHARMA SPRL

Inventor： JEAN-PASCAL PIERRE BILGISCHER ,  PHILIP JONATHAN BASSETT ,  MARK ROBERT PEARCE-HIGGINS ,  ANDREW JOHN KENNY

IPC: C07K16/00

Abstract: The present disclosure relates to a method for the manufacture of recombinant antibody molecules comprising culturing a host cell sample transformed with an expression vector encoding a recombinant antibody molecule; adding an extraction buffer to the sample; and subjecting the sample to a heat treatment step; wherein the pH of the sample is detected after addition of the extraction buffer, and optionally adjusted, to ensure that the pH of the sample is 6 to 9 prior to the heat treatment step.