公开(公告)号：US20180282783A1

公开(公告)日：2018-10-04

申请号：US15569002

申请日：2016-04-25

Applicant: UNIVERSITY OF IOWA RESEARCH FOUNDATION

Inventor： Paloma H. Giangrande ,  James O. McNamara ,  David D. Dickey ,  Howard Ozer ,  Sven Kruspe

IPC: C12Q1/44 ,  C12Q1/6823 ,  C12Q1/6886

Abstract: The present invention relates to a rapid detection of circulating tumor cell (CTC)-associated nuclease activity with chemically modified nuclease substrate probes and compositions useful in detection assays.

公开(公告)号：US20200283830A1

公开(公告)日：2020-09-10

申请号：US16794677

申请日：2020-02-19

Applicant: UNIVERSITY OF IOWA RESEARCH FOUNDATION

Inventor： James O. McNamara ,  Katie R. Flenker ,  Hyeon Kim ,  Alexander R. Howswill ,  Frank J. Hernandez ,  Mark Behlke ,  Lingyan Huang ,  Richard Owczarzy ,  Elliott Burghardt ,  Karen Clark

IPC: C12Q1/689 ,  C07H21/02 ,  C07H21/04 ,  A61K49/00 ,  C12Q1/70 ,  G01N21/64 ,  G01N33/58

Abstract: The present invention relates to a rapid detection of microbial-associated nuclease activity with chemically modified nuclease (e.g., endonuclease) substrates, and probes and compositions useful in detection assays.

公开(公告)号：US20220098648A1

公开(公告)日：2022-03-31

申请号：US17494626

申请日：2021-10-05

Applicant: UNIVERSITY OF IOWA RESEARCH FOUNDATION

Inventor： James O. McNamara ,  Katie R. Flenker ,  Hyeon Kim ,  Alexander R. Howswill ,  Frank J. Hernandez ,  Mark Behlke ,  Lingyan Huang ,  Richard Owczarzy ,  Elliott Burghardt ,  Karen Clark

IPC: C12Q1/689 ,  C07H21/02 ,  C07H21/04 ,  A61K49/00 ,  C12Q1/70 ,  G01N21/64 ,  G01N33/58

Abstract: The present invention relates to a rapid detection of microbial-associated nuclease activity with chemically modified nuclease (e.g., endonuclease) substrates, and probes and compositions useful in detection assays.

公开(公告)号：US11155882B2

公开(公告)日：2021-10-26

申请号：US16794677

申请日：2020-02-19

Applicant: UNIVERSITY OF IOWA RESEARCH FOUNDATION

Inventor： James O. McNamara ,  Katie R. Flenker ,  Hyeon Kim ,  Alexander R. Howswill ,  Frank J. Hernandez ,  Mark Behlke ,  Lingyan Huang ,  Richard Owczarzy ,  Elliott Burghardt ,  Karen Clark

IPC: C12Q1/689 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  A61K49/00 ,  C12Q1/70 ,  G01N21/64 ,  G01N33/58

Abstract: The present invention relates to a rapid detection of microbial-associated nuclease activity with chemically modified nuclease (e.g., endonuclease) substrates, and probes and compositions useful in detection assays.

公开(公告)号：US20200255883A1

公开(公告)日：2020-08-13

申请号：US16651863

申请日：2018-09-27

Applicant: UNIVERSITY OF IOWA RESEARCH FOUNDATION

Inventor： James O. McNamara

IPC: C12Q1/44 ,  C12Q1/6816

Abstract: In certain embodiments, the present invention provides a detection composition comprising a picodroplet comprising (a) an aqueous solution, and (b) a substrate probe comprising (i) an oligonucleotide of 2 to 75 nucleotides in length, (ii) a fluorophore operably linked to the oligonucleotide, and (iii) a quencher operably linked to the oligonucleotide. As used herein, the term “picodroplet” comprises a liquid droplet that has a volume of 0.014 to 2.6 picoliters. In certain embodiments, the present invention provides a method of detecting at least one individual nuclease molecule present in a sample, comprising contacting an aqueous sample suspected of containing at least one nuclease with at least one detection composition comprising a picodroplet comprising (a) an aqueous solution, and (b) a substrate probe comprising (i) an oligonucleotide of 2 to 75 nucleotides in length, (ii) a fluorophore operably linked to the oligonucleotide, and (iii) a quencher operably linked to the oligonucleotide to form an aqueous reaction mixture; emulsifying the aqueous mixture in oil to form picoliter-scale droplets in an emulsion, (c) incubating the picoliter-scale droplets in the emulsion in order for the nuclease, if present, to digest the substrate probes linked to the microbeads; recovering the microbeads; and detecting fluorescence emitting from the microbeads.