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公开(公告)号：US10030259B2
公开(公告)日：2018-07-24
申请号：US15599997
申请日：2017-05-19
Applicant: UNIVERSITY OF SOUTH CAROLINA
Inventor： Sourav Banerjee , Anindya Chanda
IPC: G01S15/89 , G01S7/52 , G01N29/06 , G01N29/22 , G01N29/34 , G01N29/46 , C12Q1/04 , A61B8/14 , A61B8/08 , A61B8/00
Abstract: Methods for ultrasonically imaging a heterogeneous 3D-cell population without physically probing are provided. The method can comprises: pulsing ultrasound waves having a wave frequency of about 10 MHz to about 2 GHz through a lens rod, wherein the lens rod focuses the ultrasound waves onto the cell population via a concave lens-head (e.g., comprising sapphire), and wherein the cell population is positioned on the reflective surface such that the ultrasound waves are reflected back to the lens-head; receiving the reflected waves through the lens head and the lens rod at a signal receiver; scanning the lens-head across multiple points in the x,y plane; and at each point in the x,y plane, moving the lens-head in a z-direction such that a signal is received at multiple intervals in the z-direction for each point in the x,y plane.
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公开(公告)号：US12108764B2
公开(公告)日：2024-10-08
申请号：US15911327
申请日：2018-03-05
Applicant: University of South Carolina
Inventor： Anindya Chanda , Jamie Lead
Abstract: Methods for inhibiting fungal secondary metabolisms are described. Secondary metabolisms inhibited by the methods can include those responsible for expression of mycotoxins such as aflatoxin. Methods can include inhibition of aflatoxin production without inhibiting fungal growth by contacting a fungus with a suspension including silver nanoparticles that can include a surface coating. The methods can be utilized in preventing fungal-caused destruction of plant species (e.g., crop species). Disclosed methods encompass utilization of silver nanoparticles at low concentrations of from about 10 picograms per liter to about 1 microgram per liter.
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公开(公告)号：US20180255784A1
公开(公告)日：2018-09-13
申请号：US15911327
申请日：2018-03-05
Applicant: University of South Carolina
Inventor： Anindya Chanda , Jamie Lead
Abstract: Methods for inhibiting fungal secondary metabolisms are described. Secondary metabolisms inhibited by the methods can include those responsible for expression of mycotoxins. Methods can include inhibition of secondary metabolisms without inhibiting fungal growth. As such, the methods can be utilized in preventing fungal-caused destruction of plant species (e.g., crop species) without undesirable consequences to plant health and local environments due to complete destruction of the fungal presence and growth. Disclosed methods encompass utilization of silver nanoparticles in inhibition of secondary metabolism at concentrations that are much lower than those typically used in toxicological and ecotoxicological studies.
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公开(公告)号：US20170253903A1
公开(公告)日：2017-09-07
申请号：US15599997
申请日：2017-05-19
Applicant: UNIVERSITY OF SOUTH CAROLINA
Inventor： Sourav Banerjee , Anindya Chanda
IPC: C12Q1/04
CPC classification number: C12Q1/04 , A61B8/14 , A61B8/44 , A61B8/483 , G01N29/0681 , G01N29/221 , G01N29/343 , G01N29/46 , G01S7/52079 , G01S15/8936 , G01S15/8956 , G01S15/8993
Abstract: Methods for ultrasonically imaging a heterogeneous 3D-cell population without physically probing are provided. The method can comprises: pulsing ultrasound waves having a wave frequency of about 10 MHz to about 2 GHz through a lens rod, wherein the lens rod focuses the ultrasound waves onto the cell population via a concave lens-head (e.g., comprising sapphire), and wherein the cell population is positioned on the reflective surface such that the ultrasound waves are reflected back to the lens-head; receiving the reflected waves through the lens head and the lens rod at a signal receiver; scanning the lens-head across multiple points in the x,y plane; and at each point in the x,y plane, moving the lens-head in a z-direction such that a signal is received at multiple intervals in the z-direction for each point in the x,y plane.
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