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公开(公告)号：US20220251517A1
公开(公告)日：2022-08-11
申请号：US17668321
申请日：2022-02-09
Applicant: University Health Network
Inventor： Sara S. Nunes de Vasconcelos , Kenneth Williams
Abstract: Various embodiments are described herein for creating a 3D human heart model for modelling arrythmias, wherein the method comprises seeding a structure with a mixture of human cardiomyocytes, cardiac fibroblasts and a fibrin mixture to form cardiac tissue; applying a plating media for settlement and compaction of the cardiac tissue; and adding an arrhythmogenic media to the cardiac tissue, where the arrhythmogenic media comprises methyl-beta cyclodextrin for disrupting calcium signaling.
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