公开(公告)号：US20220145387A1

公开(公告)日：2022-05-12

申请号：US17415586

申请日：2019-12-20

Applicant: University of Southern California

Inventor： Scott E. Fraser ,  Simon Restrepo ,  Joseph P. Dunham

IPC: C12Q1/6876 ,  C12Q1/6841 ,  C12Q1/6855

Abstract: Described herein is a method to create dendritic biocompatible polymers from pairs of complementary dendritic nucleic acid monomers in a controlled manner, using polymerization triggers. The dendritic monomers are constituted of nucleic acids and an organic polymer capable of self-assembly. A variety of additional improvements are described herein, including processes not requiring snap cooling, “wobble clamp” designs to confer a transitory measure of hairpin stability prior to branch migration, and multiple assemblies of amplifying systems. Depending on the context this technology could be used to reveal the presence of a large variety of analytes such as specific nucleic acid molecules, small molecules, proteins, and peptides.

公开(公告)号：US20230193363A1

公开(公告)日：2023-06-22

申请号：US18047215

申请日：2022-10-17

Applicant: University of Southern California

Inventor： Scott E. Fraser ,  Simon Restrepo ,  Joseph P. Dunham

IPC: C12Q1/682 ,  C12Q1/6841 ,  C12N15/11 ,  C12Q1/6834 ,  G01N21/64

CPC classification number: C12Q1/682 ,  C12Q1/6841 ,  C12N15/11 ,  C12Q1/6834 ,  G01N21/6428 ,  C12N2310/531 ,  G01N2021/6439

Abstract: Described herein is a method to create dendritic biocompatible polymers from pairs of complementary dendritic nucleic acid monomers in a controlled manner, using polymerization triggers. The dendritic monomers are constituted of nucleic acids and an organic polymer capable of self-assembly. Each polymer contains approximately 200 dendrites that can be used to attach labels and constitute a biologically compatible signal amplification technology. Depending on the context this technology could be used to reveal the presence of a large variety of analytes such as specific nucleic acid molecules, small molecules, proteins, and peptides.

公开(公告)号：US11492657B2

公开(公告)日：2022-11-08

申请号：US16606038

申请日：2018-02-28

Applicant: University of Southern California

Inventor： Scott E. Fraser ,  Simon Restrepo ,  Joseph P. Dunham

IPC: C12Q1/682 ,  C12Q1/6834 ,  C12Q1/6841 ,  C12N15/11 ,  G01N21/64

Abstract: Described herein is a method to create dendritic biocompatible polymers from pairs of complementary dendritic nucleic acid monomers in a controlled manner, using polymerization triggers. The dendritic monomers are constituted of nucleic acids and an organic polymer capable of self-assembly. Each polymer contains approximately 200 dendrites that can be used to attach labels and constitute a biologically compatible signal amplification technology. Depending on the context this technology could be used to reveal the presence of a large variety of analytes such as specific nucleic acid molecules, small molecules, proteins, and peptides.