公开(公告)号：US08802600B2

公开(公告)日：2014-08-12

申请号：US11731748

申请日：2007-03-29

Applicant: David R. Rank ,  Jeffery Wegener ,  Jonas Korlach ,  Daniel Roitman ,  Yue Xu ,  John Lyle ,  Stephen Turner ,  Paul Peluso ,  Geoff Otto ,  Ron Cicero

Inventor： David R. Rank ,  Jeffery Wegener ,  Jonas Korlach ,  Daniel Roitman ,  Yue Xu ,  John Lyle ,  Stephen Turner ,  Paul Peluso ,  Geoff Otto ,  Ron Cicero

IPC: C40B99/00

CPC classification number: C12Q1/6874 ,  B01J19/0046 ,  B01J2219/00427 ,  B01J2219/0045 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00639 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B82Y20/00 ,  B82Y30/00 ,  C12Q1/6837 ,  Y10T29/49002 ,  C12Q2565/629 ,  C12Q2527/127

Abstract: Methods of producing substrates having selected active chemical regions by employing elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate. The methods may include optical, chemical and/or mechanical processes for the deposition, removal, activation and/or deactivation of chemical groups in selected regions of the substrate to provide selective active regions of the substrate.

公开(公告)号：US08772202B2

公开(公告)日：2014-07-08

申请号：US13487978

申请日：2012-06-04

Applicant: David R. Rank ,  Jeffrey Wegener ,  Jonas Korlach ,  Daniel Roitman ,  Yue Xu ,  John Lyle ,  Stephen Turner ,  Paul Peluso ,  Geoff Otto ,  Ronald L. Cicero

Inventor： David R. Rank ,  Jeffrey Wegener ,  Jonas Korlach ,  Daniel Roitman ,  Yue Xu ,  John Lyle ,  Stephen Turner ,  Paul Peluso ,  Geoff Otto ,  Ronald L. Cicero

IPC: C40B99/00

CPC classification number: C12Q1/6874 ,  B01J19/0046 ,  B01J2219/00427 ,  B01J2219/0045 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00639 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B82Y20/00 ,  B82Y30/00 ,  C12Q1/6837 ,  Y10T29/49002 ,  C12Q2565/629 ,  C12Q2527/127

Abstract: Methods of producing substrates having selected active chemical regions by employing elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate. The methods may include optical, chemical and/or mechanical processes for the deposition, removal, activation and/or deactivation of chemical groups in selected regions of the substrate to provide selective active regions of the substrate.

Abstract translation: 通过使用底物的元素来辅助活性化学基团在基底的所需区域中的定位来制备具有选定的活性化学区域的基底的方法。 所述方法可以包括用于沉积，去除，激活和/或去活衬底的选定区域中的化学基团的光学，化学和/或机械方法，以提供衬底的选择性活性区域。

公开(公告)号：US08426217B2

公开(公告)日：2013-04-23

申请号：US13215749

申请日：2011-08-23

Applicant: David R. Walt ,  Todd A. Dickinson

Inventor： David R. Walt ,  Todd A. Dickinson

IPC: G01N21/00 ,  G01N33/543 ,  G01N21/75

CPC classification number: C12Q1/682 ,  B01J2219/00317 ,  B01J2219/0045 ,  B01J2219/00466 ,  B01J2219/005 ,  B01J2219/00524 ,  B01J2219/00585 ,  B01J2219/00648 ,  B01J2219/00659 ,  C12Q1/6834 ,  C40B60/14 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/7703 ,  G01N33/0031 ,  G01N33/54313 ,  G01N2201/0846 ,  Y10T436/101666 ,  Y10T436/109163

Abstract: Disclosed herein are compositions and methods for combining the output obtained from redundant sensor elements in a sensor array.

公开(公告)号：US20100200094A1

公开(公告)日：2010-08-12

申请号：US12702162

申请日：2010-02-08

Applicant: Sergey V. Ermakov

Inventor： Sergey V. Ermakov

IPC: F15C1/06

CPC classification number: B01L3/50273 ,  B01J19/0046 ,  B01J2219/00367 ,  B01J2219/00369 ,  B01J2219/00389 ,  B01J2219/00439 ,  B01J2219/00441 ,  B01J2219/00448 ,  B01J2219/0045 ,  B01J2219/00675 ,  B01J2219/00695 ,  B01J2219/00722 ,  B01L3/502738 ,  B01L3/502784 ,  B01L3/502792 ,  B01L7/52 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/168 ,  B01L2400/0406 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0427 ,  B01L2400/0448 ,  B01L2400/0487 ,  B01L2400/06 ,  B01L2400/0688 ,  B82Y30/00 ,  C40B40/06 ,  C40B50/14 ,  C40B60/14 ,  F15D1/00 ,  F15D1/06 ,  Y10T137/0391 ,  Y10T137/206 ,  Y10T137/2224 ,  Y10T436/115831 ,  Y10T436/12 ,  Y10T436/2575

Abstract: The present teachings relate to surface tension controlled valves used for handling biological fluids. The valves controlled by optically actuating an electro-wetting circuit.

Abstract translation: 本教导涉及用于处理生物流体的表面张力控制阀。 通过光学驱动电润湿回路控制阀。

公开(公告)号：US07083975B2

公开(公告)日：2006-08-01

申请号：US10062918

申请日：2002-02-01

Applicant: Roland Green ,  Alan Pitas ,  Francesco Cerrina

Inventor： Roland Green ,  Alan Pitas ,  Francesco Cerrina

IPC: C12M1/00

CPC classification number: B82Y30/00 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00353 ,  B01J2219/00436 ,  B01J2219/00439 ,  B01J2219/0045 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00711 ,  B01J2219/00722 ,  C40B40/06 ,  C40B50/14 ,  C40B60/14

Abstract: During the light illumination period of a monomer addition cycle in synthesizing an DNA microarray, undesirable reflections of illumination light from various interfaces that the illumination light passes through near the synthesis surface of the substrate may reduce the light-dark contrast, and negatively affect the precision and resolution of the microarray synthesis. The present invention provides an flow cell that reduces the undesired reflections by constructing certain flow cell structures with materials that have similar refractive indexes as that of the solution that is in the oligomer synthesis chamber during the illumination period and/or constructing certain flow cell structures or covering the structures with a layer of a material that has a high extinction coefficient.

公开(公告)号：US20040013569A1

公开(公告)日：2004-01-22

申请号：US10079635

申请日：2002-02-19

Inventor： Kenneth J. Balkus JR. ,  Paul Pantano ,  Claudia C. Meek ,  Decio H. Coutinho

IPC: G01N021/76

CPC classification number: G01N21/6452 ,  B01J2219/0045 ,  B01J2219/00466 ,  B01J2219/00502 ,  B01J2219/00545 ,  B01J2219/00576 ,  B01J2219/00648 ,  B01J2219/00702 ,  B01J2219/0072 ,  C40B60/14 ,  G01N21/6428 ,  G01N21/7703 ,  G01N2201/0846

Abstract: A molecular sieve particle-based analytic chemistry system is disclosed in which populations of encoded molecular sieve particles carrying different chemical functionalities are distributed into wells etched in an optical fiber bundle. The chemical functionalities are encoded on separate shaped molecular sieve particles using luminescent dyes and/or molecular sieve particle shapes and thus, a single sensor array may carry thousands of chemistries. Such encoded molecular sieve particles can provide at least a five-fold enhancement in tunable parameters for increasing the encoding possibilities of high throughput screening assays relative to the present dye-modified polymeric microsphere standard.

公开(公告)号：US20140315756A1

公开(公告)日：2014-10-23

申请号：US14318324

申请日：2014-06-27

Applicant: Pacific Biosciences of California, Inc.

Inventor： David R. Rank ,  Jeffrey Wegener ,  Jonas Korlach ,  Daniel Roitman ,  Yue Xu ,  John Lyle ,  Stephen Turner ,  Paul Peluso ,  Geoff Otto ,  Ronald L. Cicero

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  B01J19/0046 ,  B01J2219/00427 ,  B01J2219/0045 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00639 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B82Y20/00 ,  B82Y30/00 ,  C12Q1/6837 ,  Y10T29/49002 ,  C12Q2565/629 ,  C12Q2527/127

Abstract: Methods of producing substrates having selected active chemical regions by employing elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate. The methods may include optical, chemical and/or mechanical processes for the deposition, removal, activation and/or deactivation of chemical groups in selected regions of the substrate to provide selective active regions of the substrate.

Abstract translation: 通过使用底物的元素来辅助活性化学基团在基底的所需区域中的定位来制备具有选定的活性化学区域的基底的方法。 所述方法可以包括用于沉积，去除，激活和/或去活衬底的选定区域中的化学基团的光学，化学和/或机械方法，以提供衬底的选择性活性区域。

公开(公告)号：US20140249057A1

公开(公告)日：2014-09-04

申请号：US14169992

申请日：2014-01-31

Applicant: APPLIED BIOSYSTEMS, LLC

Inventor： Sergey V. Ermakov

IPC: B01L3/00 ,  B01L7/00

CPC classification number: B01L3/50273 ,  B01J19/0046 ,  B01J2219/00367 ,  B01J2219/00369 ,  B01J2219/00389 ,  B01J2219/00439 ,  B01J2219/00441 ,  B01J2219/00448 ,  B01J2219/0045 ,  B01J2219/00675 ,  B01J2219/00695 ,  B01J2219/00722 ,  B01L3/502738 ,  B01L3/502784 ,  B01L3/502792 ,  B01L7/52 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/168 ,  B01L2400/0406 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0427 ,  B01L2400/0448 ,  B01L2400/0487 ,  B01L2400/06 ,  B01L2400/0688 ,  B82Y30/00 ,  C40B40/06 ,  C40B50/14 ,  C40B60/14 ,  F15D1/00 ,  F15D1/06 ,  Y10T137/0391 ,  Y10T137/206 ,  Y10T137/2224 ,  Y10T436/115831 ,  Y10T436/12 ,  Y10T436/2575

Abstract: The present teachings provide a fluid processing device adapted to produce different oligomers in a plurality of respective reaction sites. The fluid processing device can comprise a first manifold for delivering reactants to the plurality of reaction sites, and a second manifold for removing waste from, and optionally delivering wash fluid to, the plurality of reaction sites. Surface tension control valves can be disposed in fluid communication with the first manifold and can selectively allow reactants and/or fluids into the reaction sites. A method of making oligonucleotides is also provided.

Abstract translation: 本教导提供适于在多个相应反应位点中产生不同低聚物的流体处理装置。 流体处理装置可以包括用于将反应物递送到多个反应部位的第一歧管，以及用于从多个反应部位除去废物并且可选地输送洗涤流体的第二歧管。 表面张力控制阀可以设置成与第一歧管流体连通并且可以选择性地允许反应物和/或流体进入反应位置。 还提供了制备寡核苷酸的方法。

公开(公告)号：US08193123B2

公开(公告)日：2012-06-05

申请号：US11978171

申请日：2007-10-26

Applicant: David R. Rank ,  Jeffery Wegener ,  Jonas Korlach ,  Daniel Roitman ,  Yue Xu ,  John Lyle ,  Stephen Turner ,  Paul Peluso ,  Geoff Otto ,  Ron Cicero

Inventor： David R. Rank ,  Jeffery Wegener ,  Jonas Korlach ,  Daniel Roitman ,  Yue Xu ,  John Lyle ,  Stephen Turner ,  Paul Peluso ,  Geoff Otto ,  Ron Cicero

IPC: C40B99/00

CPC classification number: C12Q1/6874 ,  B01J19/0046 ,  B01J2219/00427 ,  B01J2219/0045 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00639 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B82Y20/00 ,  B82Y30/00 ,  C12Q1/6837 ,  Y10T29/49002 ,  C12Q2565/629 ,  C12Q2527/127

Abstract: Methods of producing substrates having selected active chemical regions by employing elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate. The methods may include optical, chemical and/or mechanical processes for the deposition, removal, activation and/or deactivation of chemical groups in selected regions of the substrate to provide selective active regions of the substrate.

Abstract translation: 通过使用底物的元素来辅助活性化学基团在基底的所需区域中的定位来制备具有选定的活性化学区域的基底的方法。 所述方法可以包括用于沉积，去除，激活和/或去活衬底的选定区域中的化学基团的光学，化学和/或机械方法，以提供衬底的选择性活性区域。

公开(公告)号：US20110124524A1

公开(公告)日：2011-05-26

申请号：US12944322

申请日：2010-11-11

Applicant: Sergey V. Ermakov

Inventor： Sergey V. Ermakov

IPC: C40B40/06 ,  C40B60/14 ,  C40B50/14

CPC classification number: B01L3/50273 ,  B01J19/0046 ,  B01J2219/00367 ,  B01J2219/00369 ,  B01J2219/00389 ,  B01J2219/00439 ,  B01J2219/00441 ,  B01J2219/00448 ,  B01J2219/0045 ,  B01J2219/00675 ,  B01J2219/00695 ,  B01J2219/00722 ,  B01L3/502738 ,  B01L3/502784 ,  B01L3/502792 ,  B01L7/52 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/168 ,  B01L2400/0406 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0427 ,  B01L2400/0448 ,  B01L2400/0487 ,  B01L2400/06 ,  B01L2400/0688 ,  B82Y30/00 ,  C40B40/06 ,  C40B50/14 ,  C40B60/14 ,  F15D1/00 ,  F15D1/06 ,  Y10T137/0391 ,  Y10T137/206 ,  Y10T137/2224 ,  Y10T436/115831 ,  Y10T436/12 ,  Y10T436/2575

Abstract: The present teachings provide a fluid processing device adapted to produce different oligomers in a plurality of respective reaction sites. The fluid processing device can comprise a first manifold for delivering reactants to the plurality of reaction sites, and a second manifold for removing waste from, and optionally delivering wash fluid to, the plurality of reaction sites. Surface tension control valves can be disposed in fluid communication with the first manifold and can selectively allow reactants and/or fluids into the reaction sites. A method of making oligonucleotides is also provided.

Abstract translation: 本教导提供适于在多个相应反应位点中产生不同低聚物的流体处理装置。 流体处理装置可以包括用于将反应物递送到多个反应部位的第一歧管，以及用于从多个反应部位除去废物并且可选地输送洗涤流体的第二歧管。 表面张力控制阀可以设置成与第一歧管流体连通并且可以选择性地允许反应物和/或流体进入反应位置。 还提供了制备寡核苷酸的方法。