公开(公告)号：WO2017136344A1

公开(公告)日：2017-08-10

申请号：PCT/US2017/015842

申请日：2017-01-31

Applicant: INVISTA NORTH AMERICA S.À.R.L. ,  INVISTA TECHNOLOGIES S.À.R.L.

Inventor： BOTES, Adriana, Leonora ,  CONRADIE, Alexander, Van Eck ,  FOSTER, Alec ,  CHEN, Changlin

IPC: C12P7/04 ,  C12P7/24 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/50 ,  C12P7/64 ,  C12P13/00

CPC classification number: C12P7/40 ,  C07C47/21 ,  C07C59/147 ,  C08G69/10 ,  C08G69/26 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/80 ,  C12N9/88 ,  C12N9/90 ,  C12P7/04 ,  C12P7/24 ,  C12P7/42 ,  C12P7/46 ,  C12P7/50 ,  C12P7/6409 ,  C12P7/6427 ,  C12P13/001 ,  C12P17/182 ,  C12Y102/99006 ,  C12Y103/01031 ,  C12Y203/01032 ,  C12Y206/01018 ,  C12Y305/01 ,  C12Y402/99 ,  C12Y503/03008

Abstract: This document describes biochemical pathways for producing pimeloyl-CoA using a polypeptide having the enzymatic activity of a hydroperoxide lyase to form non-3-enal and 9- oxononanoate from 9-hydroxyperoxyoctadec-10,12-dienoate. Non-3-enal and 9-oxononanoate can be enzymatically converted to pimeloyl-CoA or a salt thereof using one or more polypeptides having the activity of a dehydrogenase , a CoA ligase , an isomerase , a reductase , a thioesterase , a monooxygenase , a hydratase , and/or a thiolase . Pimeloyl-CoA can be enzymatically converted to pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine, or 1,7-heptanediol, or corresponding salts thereof. This document also describes recombinant microorganisms producing pimeloyl-CoA, as well as pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine, and 1,7-heptanediol, or corresponding salts thereof.

Abstract translation: 该文件描述了使用具有过氧化氢裂合酶酶活性的多肽从9-氧代癸酸形成非3-烯醛和9-氧代壬酸产生庚二酰-CoA的生物化学途径， hydroxyperoxyoctadec-10,12二烯酸酯。 可使用一种或多种具有脱氢酶活性，CoA连接酶活性的多肽将非3-烯醇和9-氧代壬酸酯酶促转化为庚二酰-CoA或其盐， ，一种异构酶，一种还原酶，一种硫酯酶，一种单加氧酶，一种水合酶， ，和/或硫解酶。 庚二酰-CoA可以酶促转化为庚二酸，7-氨基庚酸，7-羟基庚酸，七亚甲基二胺或1,7-庚二醇，或其相应的盐。 该文献还描述了产生庚二酰-CoA以及庚二酸，7-氨基庚酸，7-羟基庚酸，七亚甲基二胺和1,7-庚二醇或其相应盐的重组微生物。