公开(公告)号：WO2015195682A1

公开(公告)日：2015-12-23

申请号：PCT/US2015/036057

申请日：2015-06-16

Applicant: INVISTA TECHNOLOGIES S.À.R.L.

Inventor： BOTES, Adriana Leonora ,  CONRADIE, Alex Van Eck ,  HADDOUCHE, Ramdane

IPC: C12P7/42 ,  C12P7/44

CPC classification number: C12P13/001 ,  C07C47/02 ,  C07C47/12 ,  C07C59/01 ,  C07C59/325 ,  C07C223/02 ,  C08G63/06 ,  C12N9/0008 ,  C12N9/0077 ,  C12N9/1007 ,  C12N9/1096 ,  C12P7/24 ,  C12P7/40 ,  C12P7/42 ,  C12P7/44 ,  C12P7/62 ,  C12P7/6409 ,  C12P7/6436 ,  C12Y102/99006 ,  C12Y114/15001 ,  C12Y114/15003 ,  C12Y206/01018 ,  C12Y206/01019 ,  C12Y206/01029 ,  C12Y206/01048 ,  C12Y206/01082

Abstract: This document describes biochemical pathways for producing 6- hydroxyhexanoate methyl ester and hexanoic acid hexyl ester using one or more of a fatty acid O-methyltransferase, an alcohol O-acetyltransferase and a monooxygenase , as well as recombinant hosts expressing one or more of such enzymes. 6-hydroxyhexanoate methyl esters and hexanoic acid hexyl ester can be enzymatically converted to adipic acid, adipate semialdehyde, 6-aminohexanoate, 6-hydroxyhexanoate, hexamethylenediamine, and 1,6-hexanediol.

Abstract translation: 该文献描述了使用一种或多种脂肪酸O-甲基转移酶，醇O-乙酰转移酶和单加氧酶产生6-羟基己酸甲酯和己酸己酯的生化途径，以及表达一种或多种这样的酶的重组宿主 。 6-羟基己酸甲酯和己酸己酯可以酶促转化为己二酸，己二酸半醛，6-氨基己酸，6-羟基己酸，六亚甲基二胺和1,6-己二醇。

公开(公告)号：WO2015195611A2

公开(公告)日：2015-12-23

申请号：PCT/US2015/035947

申请日：2015-06-16

Applicant: INVISTA TECHNOLOGIES S.À.R.L.

Inventor： BOTES, Adriana Leonora ,  CONRADIE, Alex Van Eck

IPC: C12P7/62 ,  C12P7/44

CPC classification number: C12P7/62 ,  C07C69/533 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/16 ,  C12N9/18 ,  C12P7/18 ,  C12P7/24 ,  C12P7/40 ,  C12P7/42 ,  C12P7/44 ,  C12P11/00 ,  C12P13/001 ,  C12P13/005 ,  C12P17/10 ,  C12Y102/99006 ,  C12Y203/01016 ,  C12Y206/01018 ,  C12Y206/01019 ,  C12Y206/01029 ,  C12Y206/01048 ,  C12Y206/01082 ,  C12Y301/01085 ,  C12Y301/02

Abstract: This document describes biochemical pathways for producing 2(E)-heptenedioyl-CoA methyl ester from precursors such as 2-oxo-glutarate, acetyl-CoA, or succinyl-CoA using one or more of a fatty acid O-methyltransferase, a thioesterase, a CoA-transferase, a CoA ligase , as well as recombinant hosts expressing one or more of such enzymes. 2(E)-heptenedioyl-CoA methyl ester can be enzymatically converted to pimeloyl-CoA using a trans-2-enoyl-CoA reductase , and a methylesterase . Pimeloyl-CoA can be enzymatically converted to pimelic acid, 7-aminoheptanoate, 7-hydroxyheptanoate, heptamethylenediamine, or 1,7-heptanediol.

Abstract translation: 本文件描述了使用一种或多种脂肪酸O-甲基转移酶，硫酯酶，硫酸酯酶等从前体例如2-氧代 - 戊二酸，乙酰辅酶A或琥珀酰-CoA制备2（E） - 庚二酰辅酶A甲酯的生化途径， CoA转移酶，CoA连接酶，以及表达一种或多种这样的酶的重组宿主。 2（E） - 庚二酰辅酶A甲酯可以使用反式-2-烯酰辅酶A还原酶和甲酯酶酶转化为双甲酰基-CoA。 可将Pimeloyl-CoA酶促转化成庚二酸，7-氨基庚酸酯，7-羟基庚酸酯，七亚甲基二胺或1,7-庚二醇。