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CN106635865B 一种用于牛鼻支原体分离纯化的培养基及其制备方法和应用 有权

一种用于牛鼻支原体分离纯化的培养基及其制备方法和应用
Abstract:
本发明公开了一种用于牛鼻支原体分离纯化的培养基，每1000ml培养基中包括有以下组分：PPLO肉汤10.5 g、脑心浸出液8.0 g、氯化钠4.0 g、硫酸镁0.05 g、氯化钾0.2 g、氯化钙0.09 g、磷酸氢二钠0.024 g、磷酸二氢钾0.03 g、葡萄糖5.0 g、水解乳蛋白5.0 g、25%新鲜酵母浸出液60 ml、无菌猪血清100 ml、无菌马血清100 ml、氨苄青霉素100 mg、10%醋酸铊1 ml；并提供了其制备方法及应用。本发明的有益效果为：本发明提供的一种用于牛鼻支原体分离纯化的培养基及其制备方法和应用，与现有技术相比，具有生长迅速和滴度高的特点，分离出牛鼻支原体的成功率高。
Abstract(English):

The invention discloses a culture medium for separation and purification of mycoplasma bovirhinis. Every 1000ml of culture medium comprises, by weight 10.5g of PPLO (pleuropneumonia-like organism) broth, 8,0g of cerebrocardiac leach liquor, 4.0g of sodium chloride, 0.05g of magnesium sulfate, 0.2g of potassium chloride, 0,09g of calcium chloride, 0.024g of disodium hydrogen phosphate, 0,03g of monopotassium phosphate, 5.0g of glucose, 5.0g of lactoalbumin hydrolysate, 60ml of 25% of fresh yeast leach liquor, 100ml of aseptic swine serum, 100ml of aseptic horse serum, 100mg of ampicillin and 1ml of 10% of thallium acetate. The invention further provides a preparation method and application of the culture medium. Compared with the prior art, the culture medium for separation and purification of the mycoplasma bovirhinis, the preparation method and the application of the culture medium have the advantages of high growing speed and titer and high mycoplasma bovirhinis separation success rate.

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