The invention provides a guiding system and method based on guide rail positioning. The guiding system based on the guide rail positioning comprises an upper computer, a guide rail band, a beacon, a receiver array or an emitting receiver array. The upper computer provides a array moving request of a receiver array or an emission reception device array; the guide rail band emits or reflects a positioning guiding signal transmitted by the emission receiver array; the positioning guiding signal is received by the receiver array or the emission receiver array; a projection of the guide rail band on the receiver array or on the emission receiver array; the receiver array and the emission receiver array are fixed on a positioned guiding target; the projection and self-positioning is compared to obtain a relative position with the guide rail band as a reference. Beacon transmission information is received to obtain an upper computer request. A beacon actual passing through sequence and a set passing through sequence is compared ro obtain a relative moving direction with the beacon as a reference; a moving request, a self-direction, speed, inclined angle information are inputted into a driving control system of a guiding target which is positined in order to realize a fact that the positioned guiding target is moved or stopped according to the requirements.