A positioning guidance system and method based on guide rails, comprising: a host computer; a guide rail band; a plurality of beacons arranged along the guide rail band; and a receiver array fixedly mounted on a target to be positioned and guided, wherein: the host computer is connected to the guide rail band and the beacons; the beacons are connected to the receiver array; the host computer transmits movement requirements and a list and sequence of beacons to be passed to the receiver array through the beacons; the guide rail band transmits a positioning guidance signal to the receiver array; and the receiver array determines a relative position of the target to be positioned and guided by taking the guide rail band as reference, according to a projection of the guide rail band on the receiver array.