The invention provides a strain detection sensor based on a graphene film with large strain coefficient and high detection sensitivity and a method of fabricating the same. The strain detection sensorcomprises a graphene film, wherein the graphene film comprises two layers of graphene monolayer films, which are overlapped with each other; and the graphene on the graphene monolayer films is distributed in an island shape. The method of fabricating the strain detection sensor based on the graphene film comprises the following steps of growing graphene monolayer films on a copper substrate; applying a binder to the surface of the graphene monolayer films to obtain a copper substrate-graphene monolayer films-adhesive film; etching the copper substrate in the copper substrate-graphene monolayer films-adhesive film to obtain a graphene monolayer films-adhesive film; lifting up the graphene monolayer films-adhesive film with the other copper substrate-graphene monolayer film to obtain a copper substrate-dual-layer graphene films-adhesive film; and finally completely etching the copper substrate in the copper substrate-dual-layer graphene films-adhesive film to obtain a dual-layer graphene films-adhesive film.