The invention discloses a steady-state malvidin derivative as well as a preparation method and application thereof. A non-thermal high-voltage pulse electric field technique is adopted to prepare malvidin in vitis amurensis through extraction and purification, and anthocyanin can be protected and prevented from damage to the maximum extent. The malvidin is subjected to ultrahigh-pressure steady-state treatment together with caffeic acid, then a malvidin derivative [mallow-3-O-guiacol (Malvidin-3-O-guiacol, Mv3C)] preparation technique is obtained, and the malvidin derivative has a stable structure, good light-heat stability and very good oxidative stress resistance functions and intestinal tract conditioning functions when being compared with malvidin without steady-state treatment.