A yeast strain and a method for the synthesis of 2,5-dihydroxymethylfuran using this strain are disclosed. The yeast strain is Meyerozyma guilliermondii SC 1103, which has been maintained in the China Center for Type Culture Collection (CCTCC, Wuhan, P.R. China) with an access No. of M2016144. The method for the synthesis of 2,5-dihydroxymethylfuran using this strain is described as follows: after pre-cultivation and cultivation, Meyerozyma guilliermondii SC 1103 cells are added into the buffer solutions containing 5-hydroxymethylfurfural and glucose; the biocatalytic reaction is conducted under designated conditions, thus affording 2,5-dihydroxymethylfuran. This disclosure has many advantages such as good selectivity, mild reaction conditions, environmental friendliness, high efficiency, and good yield.