A connection interface circuit, a memory storage device and a signal generation method are disclosed. The connection interface circuit is configured to connect a memory controller to a volatile memory module. The connection interface circuit includes a phase locking circuit, a wire module and a signal interface. The signal interface is coupled between the wire module and the memory controller. The phase locking circuit is configured to receive a first clock signal from the memory controller. The phase locking circuit is further configured to generate a second clock signal according to the first clock signal and a delay feature of the wire module. The wire module is configured to provide a third clock signal to the signal interface according to the second clock signal.