A method and a device for image producing measurement of fluorescent light, according to which a sample containing fluorophores of different species is irradiated with excitation light of at least one excitation channel defined by its spectral properties. The fluorescent light emitted by the sample is received by at least one detection channel defined by its spectral detection characteristic, and is converted into a digital signal, which is stored for further processing The properties of a number of measuring channels, respectively defined as specific combinations consisting of an excitation channel and a detection channel, are automatically set before conducting the measurement according to the result of a mathematical optimization process, which takes into account the fluorescence characteristics of at least some of the fluorophores presumed by the user to be in the sampler.