An analysis device includes a sample substance spectrum acquisition unit that acquires a plurality of sample substance spectra obtained a plurality of times for a sample substance; a storage unit that stores a plurality of reference spectra for a known substance; a first evaluation value calculation unit that calculates a first evaluation value for a combination of a sample substance spectrum and a reference spectrum extracted from the plurality of sample substance spectra and the plurality of reference spectra, the first evaluation value representing a similarity between a peak intensity ratio of the reference spectrum and a peak intensity ratio of a portion of the sample substance spectrum corresponding to a peak in the reference spectrum; and a second evaluation value calculation unit that calculates a second evaluation value representing a similarity between the sample substance and the known substance based on the first evaluation values for the combinations.