A memory interface, a memory control circuit unit, a memory storage device and a clock generation method are provided. The method includes: receiving a first data strobe signal and a second data strobe signal from a volatile memory, where the first data strobe signal and the second data strobe signal are differential signals corresponding to each other; if a relative relation between a first voltage value of the first data strobe signal and a reference voltage value of a reference voltage signal conforms to a default condition, generating a clock signal in response to the first data strobe signal and the second data strobe signal; and sampling a data signal from the volatile memory based on a raising edge and a falling edge of the clock signal. Thereby, an accuracy for sampling the data signal from the volatile memory can be improved.