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公开(公告)号：US12103996B2
公开(公告)日：2024-10-01
申请号：US16867023
申请日：2020-05-05
Applicant: Fluidigm Canada Inc.
Inventor： Mitchell A. Winnik , Mark Nitz , Vladimir Baranov , Xudong Lou
IPC: G01N33/532 , C08F8/14 , C08F8/32 , C08F8/42 , C08F220/36 , C08F220/56 , G01N33/574 , G01N33/58
CPC classification number: C08F220/36 , C08F8/14 , C08F8/32 , C08F8/42 , C08F220/56 , G01N33/532 , G01N33/57426 , G01N33/58 , Y10T436/24
Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).
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公开(公告)号：US11630050B2
公开(公告)日：2023-04-18
申请号：US17382764
申请日：2021-07-22
Applicant: Fluidigm Canada Inc.
Inventor： Alexandre Loboda
Abstract: The invention relates to methods and devices for analysis of samples using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The invention provides methods and devices in which individual ablation plumes are distinctively captured and transferred to the ICP, followed by analysis by mass cytometry.
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公开(公告)号：US11264221B2
公开(公告)日：2022-03-01
申请号：US17253538
申请日：2019-06-18
Applicant: Fluidigm Canada Inc. , Paul Corkum
Inventor： Paul Corkum , Alexander V. Loboda
IPC: H01J49/04 , G01N33/68 , G02B21/33 , H01J37/20 , H01J37/28 , H01J49/00 , H01J49/02 , H01J49/06 , H01J49/10 , H01J49/40 , H01J49/16 , H01J49/14
Abstract: The present invention relates to the high resolution imaging of samples using imaging mass spectrometry (IMS) and to the imaging of biological samples by imaging mass cytometry (IMCTM) in which labelling atoms are detected by IMS. LA-ICP-MS (a form of IMS in which the sample is ablated by a laser, the ablated material is then ionised in an inductively coupled plasma before the ions are detected by mass spectrometry) has been used for analysis of various substances, such as mineral analysis of geological samples, analysis of archaeological samples, and imaging of biological substances. However, traditional LA-ICP-MS systems and methods may not provide high resolution. Described herein are methods and systems for high resolution IMS and IMC.
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公开(公告)号：US10745743B2
公开(公告)日：2020-08-18
申请号：US16270904
申请日：2019-02-08
Applicant: Fluidigm Canada Inc.
Inventor： Olga Ornatsky
IPC: C12Q1/6825 , C12Q1/6816 , C12Q1/6841 , G01N33/58 , H01J49/00
Abstract: The invention relates to the use of inductively coupled plasma mass spectroscopy for cellular sample analysis. In some embodiments a method of performing mass spectroscopy analysis using an inductively coupled plasma mass spectroscopy system is provided. The method may include introducing a cellular sample comprising one or more cells or cellular particles into an inductively coupled plasma of the inductively coupled plasma mass spectroscopy system. The method may further include using the inductively coupled plasma mass spectroscopy system to assess the cellular sample by detecting and measuring one or more element tags in the cellular sample based on the element or isotopic compositions of the one or more element tags.
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公开(公告)号：US10577648B2
公开(公告)日：2020-03-03
申请号：US14823980
申请日：2015-08-11
Applicant: Fluidigm Canada Inc.
Inventor： Olga Ornatsky
IPC: G01N33/00 , C12Q1/6825 , C12Q1/6816 , C12Q1/6841 , G01N33/543 , G01N33/58 , G01N33/68 , H01J49/00
Abstract: The invention relates to the use of inductively coupled plasma mass spectroscopy for cellular sample analysis. In some embodiments a method of performing mass spectroscopy analysis using an inductively coupled plasma mass spectroscopy system is provided. The method may include introducing a cellular sample comprising one or more cells or cellular particles into an inductively coupled plasma of the inductively coupled plasma mass spectroscopy system. The method may further include using the inductively coupled plasma mass spectroscopy system to assess the cellular sample by detecting and measuring one or more element tags in the cellular sample based on the element or isotopic compositions of the one or more element tags.
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Patent Agency Ranking
公开(公告)号：US20180312794A1
公开(公告)日：2018-11-01
申请号：US15966432
申请日：2018-04-30
Applicant: Fluidigm Canada Inc.
Inventor： Alexander V. Loboda
CPC classification number: C12M25/00 , G01N1/286 , G01N33/4833 , G01N2001/045 , G01N2001/2886 , H01J49/0027 , H01J49/04
Abstract: Apparatus and methods for delivering biological samples to an ICP source of a mass cytometer are disclosed. Biological material is disposed on a plurality of discrete sites on a carrier. The plurality of discrete sites are configured to retain biological material and to release the biological material upon application of energy. The carrier is positioned in proximity to a gas conduit and upon release from the discrete sites, the biological material becomes entrained in a gas flow, which delivers discrete portions of biological material through the conduit to the ICP source for analysis by mass cytometry. The apparatus and methods can provide a continuous stream of discrete portions of biological material to a mass cytometer or mass spectrometer.
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公开(公告)号：US10107734B2
公开(公告)日：2018-10-23
申请号：US15231645
申请日：2016-08-08
Applicant: Fluidigm Canada Inc.
Inventor： Vladimir Baranov
IPC: G01N15/10 , G01N33/483 , G01N33/569 , G01N1/04 , C12M1/34
Abstract: A combination of mutually exclusive cell-based analytical techniques can be applied to the same group of cells for analysis. The same group of cells can be prepared for analysis by each technique resulting with candidate cells targeted for mass cytometry analysis. This configuration allows for the correlation of the information between each technique to produce a matrix of multi dimension of cellular information with the same group of cells.
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公开(公告)号：US09589779B2
公开(公告)日：2017-03-07
申请号：US14783791
申请日：2014-04-17
Applicant: Fluidigm Canada Inc.
Inventor： Alexandre Loboda
CPC classification number: G01N15/10 , G01N2015/0065 , G01N2015/1006 , H01J49/0463 , H01J49/105 , H01J49/40
Abstract: The invention relates to methods and devices for analysis of samples using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The invention provides methods and devices in which individual ablation plumes are distinctively captured and transferred to the ICP, followed by analysis by mass cytometry.
Abstract translation: 本发明涉及使用激光烧蚀电感耦合等离子体质谱（LA-ICP-MS）分析样品的方法和装置。 本发明提供方法和装置，其中个体消融羽流被明显地捕获并转移到ICP，随后通过质谱法分析。
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公开(公告)号：US20160370277A1
公开(公告)日：2016-12-22
申请号：US15253060
申请日：2016-08-31
Applicant: Fluidigm Canada Inc.
Inventor： Mitchell A. Winnik , Cedric Van-Caeyzeele , Vladimir I. Baranov
IPC: G01N15/10 , H01J49/00 , G01N33/553 , G01N33/68 , G01N33/543
CPC classification number: G01N15/1012 , G01N33/54313 , G01N33/54353 , G01N33/553 , G01N33/6848 , H01J49/0009 , Y10T436/24
Abstract: The invention relates to a new type of element encoded particles suitable for the attachment of bio molecules to enable massively multiplex bio-analytical methods, and to calibrate and tune the elemental flow cytometer mass spectrometer (FC-MS) (FIG. 1).
Abstract translation: 本发明涉及适用于附着生物分子以实现大规模多重生物分析方法以及校准和调整元流式细胞仪质谱仪（FC-MS）（图1）的新型元素编码粒子。
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公开(公告)号：US20160195466A1
公开(公告)日：2016-07-07
申请号：US14983463
申请日：2015-12-29
Applicant: Fluidigm Canada Inc.
Inventor： Alexander V. Loboda , Dmitry R. Bandura , Vladimir I. Baranov
CPC classification number: G01N15/1056 , G01N2015/0065 , G01N2015/1006 , H01J49/0009 , H01J49/025 , H01J49/04 , H01J49/105 , H01J49/40
Abstract: The inventors have improved mass cytometer to facilitate its use for the analysis of particles.
Abstract translation: 发明人已经改进了质谱仪以便于其用于分析颗粒。
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