公开(公告)号：US10669358B2

公开(公告)日：2020-06-02

申请号：US16047927

申请日：2018-07-27

Applicant: FLUIDIGM CANADA INC.

Inventor： Mitchell A. Winnik ,  Mark Nitz ,  Vladimir Baranov ,  Xudong Lou

IPC: G01N33/532 ,  C08F8/42 ,  C08F8/32 ,  C08F220/36 ,  G01N33/58 ,  C08F8/14 ,  C08F220/56 ,  G01N33/574

Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).

公开(公告)号：US10072104B2

公开(公告)日：2018-09-11

申请号：US14875553

申请日：2015-10-05

Applicant: FLUIDIGM CANADA INC.

Inventor： Mitchell A. Winnik ,  Mark Nitz ,  Vladimir Baranov ,  Xudong Lou

IPC: G01N33/574 ,  C08F220/56 ,  C08F8/32 ,  C08F8/42 ,  C08F220/36 ,  G01N33/532 ,  G01N33/58 ,  C08F8/14

CPC classification number: C08F8/32 ,  C08F8/14 ,  C08F8/42 ,  C08F220/36 ,  C08F220/56 ,  G01N33/532 ,  G01N33/57426 ,  G01N33/58 ,  Y10T436/24

Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).

公开(公告)号：US12103996B2

公开(公告)日：2024-10-01

申请号：US16867023

申请日：2020-05-05

Applicant: Fluidigm Canada Inc.

Inventor： Mitchell A. Winnik ,  Mark Nitz ,  Vladimir Baranov ,  Xudong Lou

IPC: G01N33/532 ,  C08F8/14 ,  C08F8/32 ,  C08F8/42 ,  C08F220/36 ,  C08F220/56 ,  G01N33/574 ,  G01N33/58

CPC classification number: C08F220/36 ,  C08F8/14 ,  C08F8/32 ,  C08F8/42 ,  C08F220/56 ,  G01N33/532 ,  G01N33/57426 ,  G01N33/58 ,  Y10T436/24

Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).

公开(公告)号：US09296838B2

公开(公告)日：2016-03-29

申请号：US14659224

申请日：2015-03-16

Applicant: FLUIDIGM CANADA INC.

Inventor： Mitchell A. Winnik ,  Mark Nitz ,  Vladimir Baranov ,  Xudong Lou

IPC: G01N33/544 ,  G01N33/545 ,  G01N33/24 ,  C08F8/32 ,  C08F8/42 ,  C08F220/36 ,  G01N33/532 ,  G01N33/58 ,  C08F8/14

CPC classification number: C08F8/32 ,  C08F8/14 ,  C08F8/42 ,  C08F220/36 ,  C08F220/56 ,  G01N33/532 ,  G01N33/57426 ,  G01N33/58 ,  Y10T436/24

Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).

公开(公告)号：US10752707B2

公开(公告)日：2020-08-25

申请号：US16506243

申请日：2019-07-09

Applicant: Fluidigm Canada Inc.

Inventor： Mitchell A. Winnik ,  Mark Nitz ,  Vladimir Baranov ,  Xudong Lou

IPC: G01N33/532 ,  C08F8/42 ,  C08F8/32 ,  C08F220/36 ,  G01N33/58 ,  C08F8/14 ,  C08F220/56 ,  G01N33/574

Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).

公开(公告)号：US09169335B2

公开(公告)日：2015-10-27

申请号：US14659224

申请日：2015-03-16

Applicant: FLUIDIGM CANADA INC.

Inventor： Mitchell A. Winnik ,  Mark Nitz ,  Vladimir Baranov ,  Xudong Lou

IPC: G01N33/544 ,  G01N33/545 ,  G01N33/24 ,  C08F8/32 ,  C08F8/14 ,  C08F8/42

Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).