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    Polymer backbone element tags
    2.
    发明授权
    Polymer backbone element tags 审中-公开

    公开(公告)号：US10669358B2

    公开(公告)日：2020-06-02

    申请号：US16047927

    申请日：2018-07-27

    Applicant: FLUIDIGM CANADA INC.

    Inventor： Mitchell A. Winnik ,  Mark Nitz ,  Vladimir Baranov ,  Xudong Lou

    IPC: G01N33/532 ,  C08F8/42 ,  C08F8/32 ,  C08F220/36 ,  G01N33/58 ,  C08F8/14 ,  C08F220/56 ,  G01N33/574

    Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).

    Polymer backbone element tags
    4.
    发明授权
    Polymer backbone element tags 有权

    公开(公告)号：US10752707B2

    公开(公告)日：2020-08-25

    申请号：US16506243

    申请日：2019-07-09

    Applicant: Fluidigm Canada Inc.

    Inventor： Mitchell A. Winnik ,  Mark Nitz ,  Vladimir Baranov ,  Xudong Lou

    IPC: G01N33/532 ,  C08F8/42 ,  C08F8/32 ,  C08F220/36 ,  G01N33/58 ,  C08F8/14 ,  C08F220/56 ,  G01N33/574

    Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).

    Polymer backbone element tags
    5.
    发明授权
    Polymer backbone element tags 有权

    公开(公告)号：US09169335B2

    公开(公告)日：2015-10-27

    申请号：US14659224

    申请日：2015-03-16

    Applicant: FLUIDIGM CANADA INC.

    Inventor： Mitchell A. Winnik ,  Mark Nitz ,  Vladimir Baranov ,  Xudong Lou

    IPC: G01N33/544 ,  G01N33/545 ,  G01N33/24 ,  C08F8/32 ,  C08F8/14 ,  C08F8/42

    Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).

    Cell analysis by mass cytometry
    7.
    发明授权
    Cell analysis by mass cytometry 有权

    公开(公告)号：US10107734B2

    公开(公告)日：2018-10-23

    申请号：US15231645

    申请日：2016-08-08

    Applicant: Fluidigm Canada Inc.

    Inventor： Vladimir Baranov

    IPC: G01N15/10 ,  G01N33/483 ,  G01N33/569 ,  G01N1/04 ,  C12M1/34

    Abstract: A combination of mutually exclusive cell-based analytical techniques can be applied to the same group of cells for analysis. The same group of cells can be prepared for analysis by each technique resulting with candidate cells targeted for mass cytometry analysis. This configuration allows for the correlation of the information between each technique to produce a matrix of multi dimension of cellular information with the same group of cells.

    CELL ANALYSIS BY MASS CYTOMETRY
    8.
    发明申请
    CELL ANALYSIS BY MASS CYTOMETRY 审中-公开
    细胞分析通过细胞计数

    公开(公告)号：US20160131635A1

    公开(公告)日：2016-05-12

    申请号：US15001589

    申请日：2016-01-20

    Applicant: Fluidigm Canada Inc.

    Inventor： Vladimir Baranov

    IPC: G01N33/483 ,  G01N15/10

    CPC classification number: G01N15/10 ,  C12M1/343 ,  G01N1/04 ,  G01N33/4833 ,  G01N33/569 ,  G01N2001/045 ,  G01N2015/1006 ,  G01N2015/1093

    Abstract: A combination of mutually exclusive cell-based analytical techniques can be applied to the same group of cells for analysis. The same group of cells can be prepared for analysis by each technique resulting with candidate cells targeted for mass cytometry analysis. This configuration allows for the correlation of the information between each technique to produce a matrix of multi dimension of cellular information with the same group of cells.

    Abstract translation: 相互排斥的基于细胞的分析技术的组合可以应用于同一组细胞用于分析。 可以通过用针对质粒细胞分析的候选细胞产生的每种技术来制备相同组的细胞用于分析。 该配置允许每个技术之间的信息的相关性，以产生具有相同组的单元的蜂窝信息的多维矩阵。

    Stabilized cell acquisition for elemental analysis
    10.
    发明授权
    Stabilized cell acquisition for elemental analysis 有权

    公开(公告)号：US12013391B2

    公开(公告)日：2024-06-18

    申请号：US16383241

    申请日：2019-04-12

    Applicant: Fluidigm Canada Inc.

    Inventor： Vladimir Baranov ,  Alexander Loboda ,  Michael Sullivan

    IPC: G01N33/53 ,  G01N33/58 ,  H01J49/10

    CPC classification number: G01N33/5306 ,  G01N33/58 ,  H01J49/105 ,  G01N2458/15 ,  G01N2560/00

    Abstract: Analyzing samples injected into an inductively coupled plasma source can be improved by one or more of a stabilizing solution mixable with a sample prior to injection and a heated injector. The stabilizing solution can minimize the difference in osmotic pressure between the solution and the cells with a relatively low amount of dissolved solids (e.g., at or below about 0.2%). The stabilizing solution can contain a salt (e.g., ammonium nitrate) present in concentrations of at least 5 mM. The injector can be heated before and/or during injection. In some cases, heat from adjacent parts can be channeled into the injector to improve heating of the injector. An injector heated to sufficient temperatures can minimize solute buildup and can extend the usable time between cleanings. These improvements can be especially useful in elemental analysis, such as inductively coupled plasma mass spectrometry or inductively coupled plasma optical emission spectrometry.

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