公开(公告)号：WO2005017499A8

公开(公告)日：2006-07-06

申请号：PCT/US2004026225

申请日：2004-08-13

Applicant: LUMINEX CORP ,  ROTH WAYNE D ,  MOORE DOUGLAS E

Inventor： ROTH WAYNE D ,  MOORE DOUGLAS E

IPC: G01N15/14

CPC classification number: G01N15/1404 ,  G01N15/1012 ,  G01N21/645 ,  G01N2015/1415 ,  G01N2015/1477 ,  G01N2201/12746

Abstract: Various methods for controlling one or more parameters of a flow cytometer type measurement system are provided. One embodiment includes monitoring parameter(s) of the measurement system during measurements of sample microspheres. The method also includes altering the parameter(s) in real time based on the monitoring. Another method includes monitoring a temperature proximate to the measurement system. One such method includes altering a bias voltage of an avalanche photo diode in response to the temperature using empirically derived data. A different such method includes altering output signals of a photomultiplier tube in response to the temperature using a characteristic curve. Some methods include monitoring a temperature of a fluid, in which sample microspheres are disposed, that will flow through the flow cytometer type measurement system. This method also includes determining a velocity of the sample microspheres in the measurement system from a viscosity of the fluid at the temperature.

Abstract translation: 提供了用于控制流式细胞仪型测量系统的一个或多个参数的各种方法。 一个实施例包括在样品微球的测量期间监测测量系统的参数。 该方法还包括基于监视实时更改参数。 另一种方法包括监测靠近测量系统的温度。 一种这样的方法包括使用经验导出的数据来响应于温度来改变雪崩光电二极管的偏置电压。 不同的这种方法包括使用特性曲线响应于温度改变光电倍增管的输出信号。 一些方法包括监测将流过流式细胞仪类型测量系统的流体样品微球的设置温度。 该方法还包括从该温度下的流体的粘度确定测量系统中的样品微球的速度。

公开(公告)号：WO2005068971A1

公开(公告)日：2005-07-28

申请号：PCT/US2005/001866

申请日：2005-01-13

Applicant: LUMINEX CORPORATION ,  ROTH, Wayne, D.

Inventor： ROTH, Wayne, D.

IPC: G01N15/14

CPC classification number: B82Y20/00 ,  B82Y10/00 ,  G01N15/14

Abstract: Methods and systems for expanding the dynamic range of a system are provided. One method includes splitting fluorescent light emitted by a particle into multiple light paths having different intensities, detecting the fluorescent light in the multiple light paths with different channels to generate multiple signals, and determining which of the channels is operating in a linear range based on the multiple signals. The method also includes altering the signal generated by the channel operating in the linear rang to compensate for the different intensities. Another method includes illuminating a particle in multiple illumination zones with light having different intensities and separately detecting fluorescent light emitted by the particle while located in the multiple illumination zones to generate multiple signals. The method also includes determining which of the signals is located in a linear range and altering the signal located in the linear range to compensate for the different intensities.

Abstract translation: 提供了扩展系统动态范围的方法和系统。 一种方法包括将由颗粒发射的荧光分成具有不同强度的多个光路，检测具有不同通道的多个光路中的荧光以产生多个信号，以及基于以下步骤确定哪个通道在线性范围内操作 多个信号。 该方法还包括改变由在线性范围中操作的通道产生的信号以补偿不同的强度。 另一种方法包括用具有不同强度的光照射多个照明区域中的颗粒，并分别检测位于多个照明区域中的颗粒发射的荧光，以产生多个信号。 该方法还包括确定哪个信号位于线性范围内并且改变位于线性范围内的信号以补偿不同的强度。

公开(公告)号：WO2010009424A2

公开(公告)日：2010-01-21

申请号：PCT/US2009/051039

申请日：2009-07-17

Applicant: LUMINEX CORPORATION ,  ROTH, Wayne, D.

Inventor： ROTH, Wayne, D.

IPC: G06F17/00 ,  G01N35/00

CPC classification number: G06K9/00147

Abstract: Methods and systems are provided which include configurations for the reassigning unit locations of a classification matrix at which two or more classification regions overlap as non-classification regions. In addition, methods and systems are provided which include configurations for mathematically creating classification regions which may be characterized by values which more accurately correspond to measured values of particles. Other embodiments of methods and systems include configurations for acquiring data corresponding to measurable parameters of a particle and identifying a location within a classification matrix to which at least some of the data corresponds. Such methods and systems further include configurations for translating either the data corresponding to the identified unit location or a target space located at known locations within the classification matrix a preset number of predetermined coordinate paths until a conclusion that the particle may be classified to particular particle category or a reject class is attained.

Abstract translation: 提供了方法和系统，其包括用于将两个或更多个分类区域重叠为非分类区域的分类矩阵的重新分配单元位置的配置。 此外，提供了包括用于在数学上创建分类区域的配置的方法和系统，所述分类区域可以通过更精确地对应于粒子的测量值的值来表征分类区域。 方法和系统的其他实施例包括用于获取与粒子的可测量参数相对应的数据并且识别至少一些数据对应于的分类矩阵内的位置的配置。 这样的方法和系统还包括用于将与识别的单元位置相对应的数据或位于分类矩阵内的已知位置处的目标空间的数据翻译预设数量的预定坐标路径，直到可以将粒子归类为特定粒子类别 或拒绝课程获得。

公开(公告)号：WO2008061058B1

公开(公告)日：2008-09-12

申请号：PCT/US2007084417

申请日：2007-11-12

Applicant: LUMINEX CORP ,  KRAGER JARDEN E ,  DEICHER WILLIAM R ,  SCHILFFARTH ADAM RICHARD ,  PEMPSELL PAUL ,  ROTH WAYNE D

Inventor： KRAGER JARDEN E ,  DEICHER WILLIAM R ,  SCHILFFARTH ADAM RICHARD ,  PEMPSELL PAUL ,  ROTH WAYNE D

IPC: G01N15/14

CPC classification number: G01N15/1404 ,  G01N21/6428 ,  G01N21/76 ,  G01N2015/1409 ,  Y10T137/0352 ,  Y10T137/85986 ,  Y10T436/2575

Abstract: A flow cytometer is provided which includes an interrogation flow cell and a plurality of assay fluidic lines extending into the interrogation flow cell. A method of operating such a flow cytometer includes priming the interrogation flow cell with a sheath fluid and injecting different assay fluids into a flow of the sheath fluid through the plurality of fluidic lines. A fluidic line assembly is provided which includes a plurality of capillary tubes coupled to a base section configured for coupling to an interrogation flow cell assembly of a flow cytometer. The capillary tubes are dimensionally configured such that when the fluidic line assembly is arranged within the flow cytometer and fluid is dispensed from one or more of the capillary tubes at a given pressure differential with respect to an encompassing sheath fluid within the interrogation flow cell the fluid is substantially centrally aligned within the interrogation flow cell.

Abstract translation: 提供流式细胞仪，其包括询问流动池和延伸到询问流动池中的多个测定流体线。 操作这种流式细胞仪的方法包括用鞘液灌注询问流动池并将不同的化验流体注入通过多条流体管线的鞘液流中。 提供流体管线组件，其包括耦合到基部的多个毛细管，所述基部被配置为耦合到流式细胞仪的询问流动池组件。 毛细管在尺寸上构造成使得当流体管线组件布置在流式细胞仪内并且流体从相对于询问流动池内的包围鞘液的给定压差从一个或多个毛细管分配时，流体 基本上在询问流动单元内居中对齐。

公开(公告)号：WO2007143615A3

公开(公告)日：2007-12-13

申请号：PCT/US2007/070345

申请日：2007-06-04

Applicant: LUMINEX CORPORATION ,  ROTH, Wayne, D. ,  COLLINS, Charles, J. ,  DUONG, Dung

Inventor： ROTH, Wayne, D. ,  COLLINS, Charles, J. ,  DUONG, Dung

IPC: G01N33/543

Abstract: Systems and methods for performing measurements of one or more materials are provided. One system is configured to transfer one or more materials to an imaging volume of a measurement device from one or more storage vessels. Another system is configured to image one or more materials in an imaging volume of a measurement device. An additional system is configured to substantially immobilize one or more materials in an imaging volume of a measurement device. A further system is configured to transfer one or more materials to an imaging volume of a measurement device from one or more storage vessels, to image the one or more materials in the imaging volume, to substantially immobilize the one or more materials in the imaging volume, or some combination thereof.

公开(公告)号：WO2007016517A1

公开(公告)日：2007-02-08

申请号：PCT/US2006/029806

申请日：2006-08-01

Applicant: LUMINEX CORPORATION ,  CALVIN, Edward, A. ,  ROTH, Wayne, D.

Inventor： CALVIN, Edward, A. ,  ROTH, Wayne, D.

IPC: G06F19/00 ,  G01N15/14

CPC classification number: G01N15/1456 ,  G01N2015/1477 ,  G01N2015/1488 ,  G06F19/707

Abstract: Methods, data structures, and systems for classifying particles are provided. In particular, the methods and systems are configured to acquire a first set of data corresponding to measurable parameters of a microparticle and identify a location of a look-up table to which the first set of data corresponds, wherein the look-up table is framed by values associated with at least one of the measurable parameters. Furthermore, the methods and systems are configured to determine whether the first set of data fits one or more predefined algorithms respectively indicative of a different microparticle classification associated with the identified location of the look-up table. The methods and systems are further configured to classifying the microparticle within at least one predefined categorization based upon the determination of whether the first set of data fits the one or more predefined algorithms.

Abstract translation: 提供了分类粒子的方法，数据结构和系统。 特别地，方法和系统被配置为获取对应于微粒的可测量参数的第一组数据，并且识别第一组数据对应的查找表的位置，其中查找表被框架 通过与至少一个可测量参数相关联的值。 此外，方法和系统被配置为确定第一组数据是否适合分别指示与查找表的所识别的位置相关联的不同微粒分类的一个或多个预定义算法。 所述方法和系统还被配置为基于确定所述第一组数据是否符合所述一个或多个预定义算法来确定所述至少一个预定义分类中的所述微粒。

公开(公告)号：WO2010009424A3

公开(公告)日：2010-04-01

申请号：PCT/US2009051039

申请日：2009-07-17

Applicant: LUMINEX CORP ,  ROTH WAYNE D

Inventor： ROTH WAYNE D

IPC: G06F17/00 ,  G01N35/00

CPC classification number: G06K9/00147

Abstract: Methods and systems are provided which include configurations for the reassigning unit locations of a classification matrix at which two or more classification regions overlap as non-classification regions. In addition, methods and systems are provided which include configurations for mathematically creating classification regions which may be characterized by values which more accurately correspond to measured values of particles. Other embodiments of methods and systems include configurations for acquiring data corresponding to measurable parameters of a particle and identifying a location within a classification matrix to which at least some of the data corresponds. Such methods and systems further include configurations for translating either the data corresponding to the identified unit location or a target space located at known locations within the classification matrix a preset number of predetermined coordinate paths until a conclusion that the particle may be classified to particular particle category or a reject class is attained.

Abstract translation: 提供了包括用于两个或多个分类区域作为非分类区域重叠的分类矩阵的重新分配单元位置的配置的方法和系统。 此外，提供了方法和系统，其包括用于数学地创建分类区域的配置，其可以通过更精确地对应于颗粒的测量值的值来表征。 方法和系统的其他实施例包括用于获取对应于粒子的可测量参数的数据的配置，并且识别至少部分数据对应的分类矩阵内的位置。 这样的方法和系统还包括用于将对应于所识别的单元位置的数据或位于分类矩阵内的已知位置处的预定数量的预定坐标路径的目标空间进行翻译的配置，直到可以将粒子分类为特定粒子类别 或获得拒绝类。

公开(公告)号：WO2007143615B1

公开(公告)日：2008-05-08

申请号：PCT/US2007070345

申请日：2007-06-04

Applicant: LUMINEX CORP ,  ROTH WAYNE D ,  COLLINS CHARLES J ,  DUONG DUNG

Inventor： ROTH WAYNE D ,  COLLINS CHARLES J ,  DUONG DUNG

IPC: G01N33/543

CPC classification number: G01N33/54333

Abstract: Systems and methods for performing measurements of one or more materials are provided. One system is configured to transfer one or more materials to an imaging volume of a measurement device from one or more storage vessels. Another system is configured to image one or more materials in an imaging volume of a measurement device. An additional system is configured to substantially immobilize one or more materials in an imaging volume of a measurement device. A further system is configured to transfer one or more materials to an imaging volume of a measurement device from one or more storage vessels, to image the one or more materials in the imaging volume, to substantially immobilize the one or more materials in the imaging volume, or some combination thereof.

Abstract translation: 提供了用于执行一种或多种材料的测量的系统和方法。 一个系统被配置为将一种或多种材料从一个或多个存储容器传送到测量装置的成像体积。 另一系统被配置成对测量装置的成像体积中的一种或多种材料进行成像。 附加系统被配置为基本上将一种或多种材料固定在测量装置的成像体积中。 另一系统被配置成将一种或多种材料从一个或多个存储容器传送到测量装置的成像体积，以对成像体积中的一种或多种材料进行成像，以使成像体积中的一种或多种材料基本上固定 ，或其某些组合。

公开(公告)号：WO2007143615A2

公开(公告)日：2007-12-13

申请号：PCT/US2007070345

申请日：2007-06-04

Applicant: LUMINEX CORP ,  ROTH WAYNE D ,  COLLINS CHARLES J ,  DUONG DUNG

Inventor： ROTH WAYNE D ,  COLLINS CHARLES J ,  DUONG DUNG

IPC: G01N33/543 ,  G01N21/64

CPC classification number: G01N33/54333

Abstract: Systems and methods for performing measurements of one or more materials are provided. One system is configured to transfer one or more materials to an imaging volume of a measurement device from one or more storage vessels. Another system is configured to image one or more materials in an imaging volume of a measurement device. An additional system is configured to substantially immobilize one or more materials in an imaging volume of a measurement device. A further system is configured to transfer one or more materials to an imaging volume of a measurement device from one or more storage vessels, to image the one or more materials in the imaging volume, to substantially immobilize the one or more materials in the imaging volume, or some combination thereof.

Abstract translation: 提供了用于执行一种或多种材料的测量的系统和方法。 一个系统被配置为将一种或多种材料从一个或多个存储容器传送到测量装置的成像体积。 另一系统被配置成对测量装置的成像体积中的一种或多种材料进行成像。 附加系统被配置为基本上将一种或多种材料固定在测量装置的成像体积中。 另一系统被配置成将一种或多种材料从一个或多个存储容器传送到测量装置的成像体积，以对成像体积中的一种或多种材料进行成像，以使成像体积中的一种或多种材料基本上固定 ，或其某些组合。

公开(公告)号：WO2007035840A2

公开(公告)日：2007-03-29

申请号：PCT/US2006/036733

申请日：2006-09-21

Applicant: LUMINEX CORPORATION ,  ROTH, Wayne, D.

Inventor： ROTH, Wayne, D.

IPC: G01N15/14

CPC classification number: G06K9/0014 ,  G01N15/1463 ,  G06T7/0012 ,  G06T7/194 ,  G06T2207/10064 ,  G06T2207/20021 ,  G06T2207/30024 ,  G06T2207/30072

Abstract: Methods, storage mediums, and systems for image data processing are provided. Embodiments for the methods, storage mediums, and systems include configurations to perform one or more of the following steps: background signal measurement, particle identification using classification dye emission and cluster rejection, inter-image alignment, inter-image particle correlation, fluorescence integration of reporter emission, and image plane normalization.

Abstract translation: 提供了用于图像数据处理的方法，存储介质和系统。 用于该方法，存储介质和系统的实施例包括执行以下步骤中的一个或多个的配置：背景信号测量，使用分类染料发射和簇拒绝的粒子识别，图像间对齐，图像间粒子相关， 记者发射和图像平面归一化。