公开(公告)号：WO2005017499A8

公开(公告)日：2006-07-06

申请号：PCT/US2004026225

申请日：2004-08-13

Applicant: LUMINEX CORP ,  ROTH WAYNE D ,  MOORE DOUGLAS E

Inventor： ROTH WAYNE D ,  MOORE DOUGLAS E

IPC: G01N15/14

CPC classification number: G01N15/1404 ,  G01N15/1012 ,  G01N21/645 ,  G01N2015/1415 ,  G01N2015/1477 ,  G01N2201/12746

Abstract: Various methods for controlling one or more parameters of a flow cytometer type measurement system are provided. One embodiment includes monitoring parameter(s) of the measurement system during measurements of sample microspheres. The method also includes altering the parameter(s) in real time based on the monitoring. Another method includes monitoring a temperature proximate to the measurement system. One such method includes altering a bias voltage of an avalanche photo diode in response to the temperature using empirically derived data. A different such method includes altering output signals of a photomultiplier tube in response to the temperature using a characteristic curve. Some methods include monitoring a temperature of a fluid, in which sample microspheres are disposed, that will flow through the flow cytometer type measurement system. This method also includes determining a velocity of the sample microspheres in the measurement system from a viscosity of the fluid at the temperature.

Abstract translation: 提供了用于控制流式细胞仪型测量系统的一个或多个参数的各种方法。 一个实施例包括在样品微球的测量期间监测测量系统的参数。 该方法还包括基于监视实时更改参数。 另一种方法包括监测靠近测量系统的温度。 一种这样的方法包括使用经验导出的数据来响应于温度来改变雪崩光电二极管的偏置电压。 不同的这种方法包括使用特性曲线响应于温度改变光电倍增管的输出信号。 一些方法包括监测将流过流式细胞仪类型测量系统的流体样品微球的设置温度。 该方法还包括从该温度下的流体的粘度确定测量系统中的样品微球的速度。

公开(公告)号：WO2008061058B1

公开(公告)日：2008-09-12

申请号：PCT/US2007084417

申请日：2007-11-12

Applicant: LUMINEX CORP ,  KRAGER JARDEN E ,  DEICHER WILLIAM R ,  SCHILFFARTH ADAM RICHARD ,  PEMPSELL PAUL ,  ROTH WAYNE D

Inventor： KRAGER JARDEN E ,  DEICHER WILLIAM R ,  SCHILFFARTH ADAM RICHARD ,  PEMPSELL PAUL ,  ROTH WAYNE D

IPC: G01N15/14

CPC classification number: G01N15/1404 ,  G01N21/6428 ,  G01N21/76 ,  G01N2015/1409 ,  Y10T137/0352 ,  Y10T137/85986 ,  Y10T436/2575

Abstract: A flow cytometer is provided which includes an interrogation flow cell and a plurality of assay fluidic lines extending into the interrogation flow cell. A method of operating such a flow cytometer includes priming the interrogation flow cell with a sheath fluid and injecting different assay fluids into a flow of the sheath fluid through the plurality of fluidic lines. A fluidic line assembly is provided which includes a plurality of capillary tubes coupled to a base section configured for coupling to an interrogation flow cell assembly of a flow cytometer. The capillary tubes are dimensionally configured such that when the fluidic line assembly is arranged within the flow cytometer and fluid is dispensed from one or more of the capillary tubes at a given pressure differential with respect to an encompassing sheath fluid within the interrogation flow cell the fluid is substantially centrally aligned within the interrogation flow cell.

Abstract translation: 提供流式细胞仪，其包括询问流动池和延伸到询问流动池中的多个测定流体线。 操作这种流式细胞仪的方法包括用鞘液灌注询问流动池并将不同的化验流体注入通过多条流体管线的鞘液流中。 提供流体管线组件，其包括耦合到基部的多个毛细管，所述基部被配置为耦合到流式细胞仪的询问流动池组件。 毛细管在尺寸上构造成使得当流体管线组件布置在流式细胞仪内并且流体从相对于询问流动池内的包围鞘液的给定压差从一个或多个毛细管分配时，流体 基本上在询问流动单元内居中对齐。

公开(公告)号：WO2007035840A3

公开(公告)日：2007-10-04

申请号：PCT/US2006036733

申请日：2006-09-21

Applicant: LUMINEX CORP ,  ROTH WAYNE D

Inventor： ROTH WAYNE D

IPC: G01N15/14

CPC classification number: G06K9/0014 ,  G01N15/1463 ,  G06T7/0012 ,  G06T7/194 ,  G06T2207/10064 ,  G06T2207/20021 ,  G06T2207/30024 ,  G06T2207/30072

Abstract: Methods, storage mediums, and systems for image data processing are provided. Embodiments for the methods, storage mediums, and systems include configurations to perform one or more of the following steps: background signal measurement, particle identification using classification dye emission and cluster rejection, inter-image alignment, inter-image particle correlation, fluorescence integration of reporter emission, and image plane normalization.

Abstract translation: 提供了用于图像数据处理的方法，存储介质和系统。 用于方法，存储介质和系统的实施例包括执行以下一个或多个步骤的配置：背景信号测量，使用分类染料发射和簇排斥的粒子识别，图像间对准，图像间粒子相关，荧光整合 记者发射和图像平面归一化。

公开(公告)号：WO2007016517B1

公开(公告)日：2007-04-05

申请号：PCT/US2006029806

申请日：2006-08-01

Applicant: LUMINEX CORP ,  CALVIN EDWARD A ,  ROTH WAYNE D

Inventor： CALVIN EDWARD A ,  ROTH WAYNE D

IPC: G01N15/14 ,  G06F19/00

CPC classification number: G01N15/1456 ,  G01N2015/1477 ,  G01N2015/1488 ,  G06F19/707

Abstract: Methods, data structures, and systems for classifying particles are provided. In particular, the methods and systems are configured to acquire a first set of data corresponding to measurable parameters of a microparticle and identify a location of a look-up table to which the first set of data corresponds, wherein the look-up table is framed by values associated with at least one of the measurable parameters. Furthermore, the methods and systems are configured to determine whether the first set of data fits one or more predefined algorithms respectively indicative of a different microparticle classification associated with the identified location of the look-up table. The methods and systems are further configured to classifying the microparticle within at least one predefined categorization based upon the determination of whether the first set of data fits the one or more predefined algorithms.

Abstract translation: 提供了用于分类粒子的方法，数据结构和系统。 具体而言，所述方法和系统被配置为获取与微粒的可测量参数相对应的第一组数据，并识别与第一组数据对应的查找表的位置，其中查找表被构建 通过与至少一个可测量参数相关联的值。 此外，方法和系统被配置为确定第一组数据是否适合分别指示与查找表的识别位置相关联的不同微粒分类的一个或多个预定义算法。 所述方法和系统进一步被配置为基于确定所述第一组数据是否适合所述一个或多个预定义算法来将所述微粒分类在至少一个预定义分类内。

公开(公告)号：WO2008061058A3

公开(公告)日：2008-07-03

申请号：PCT/US2007084417

申请日：2007-11-12

Applicant: LUMINEX CORP ,  KRAGER JARDEN E ,  DEICHER WILLIAM R ,  SCHILFFARTH ADAM RICHARD ,  PEMPSELL PAUL ,  ROTH WAYNE D

Inventor： KRAGER JARDEN E ,  DEICHER WILLIAM R ,  SCHILFFARTH ADAM RICHARD ,  PEMPSELL PAUL ,  ROTH WAYNE D

IPC: G01N15/14

CPC classification number: G01N15/1404 ,  G01N21/6428 ,  G01N21/76 ,  G01N2015/1409 ,  Y10T137/0352 ,  Y10T137/85986 ,  Y10T436/2575

Abstract: A flow cytometer is provided which includes an interrogation flow cell and a plurality of assay fluidic lines extending into the interrogation flow cell. A method of operating such a flow cytometer includes priming the interrogation flow cell with a sheath fluid and injecting different assay fluids into a flow of the sheath fluid through the plurality of fluidic lines. A fluidic line assembly is provided which includes a plurality of capillary tubes coupled to a base section configured for coupling to an interrogation flow cell assembly of a flow cytometer. The capillary tubes are dimensionally configured such that when the fluidic line assembly is arranged within the flow cytometer and fluid is dispensed from one or more of the capillary tubes at a given pressure differential with respect to an encompassing sheath fluid within the interrogation flow cell the fluid is substantially centrally aligned within the interrogation flow cell.

公开(公告)号：WO2007035840B1

公开(公告)日：2007-12-06

申请号：PCT/US2006036733

申请日：2006-09-21

Applicant: LUMINEX CORP ,  ROTH WAYNE D

Inventor： ROTH WAYNE D

IPC: G01N15/14

CPC classification number: G06K9/0014 ,  G01N15/1463 ,  G06T7/0012 ,  G06T7/194 ,  G06T2207/10064 ,  G06T2207/20021 ,  G06T2207/30024 ,  G06T2207/30072

Abstract: Methods, storage mediums, and systems for image data processing are provided. Embodiments for the methods, storage mediums, and systems include configurations to perform one or more of the following steps: background signal measurement, particle identification using classification dye emission and cluster rejection, inter-image alignment, inter-image particle correlation, fluorescence integration of reporter emission, and image plane normalization.

Abstract translation: 提供了用于图像数据处理的方法，存储介质和系统。 用于该方法，存储介质和系统的实施例包括执行以下步骤中的一个或多个的配置：背景信号测量，使用分类染料发射和簇拒绝的粒子识别，图像间对齐，图像间粒子相关， 记者发射和图像平面归一化。

公开(公告)号：WO2005017498B1

公开(公告)日：2006-06-01

申请号：PCT/US2004024987

申请日：2004-08-03

Applicant: LUMINEX CORP ,  ROTH WAYNE D

Inventor： ROTH WAYNE D

IPC: G01N15/14

CPC classification number: G01N15/1434

Abstract: Various light emitting diode (LED) based measurement systems and methods are provided. One system includes one or more arrays of LEDs (14, 16, 18) arranged along a flow path (20) of a sample (10). The array(s) are configured to illuminate the sample as the sample moves along the flow path. The system also includes one or more detectors (24) configured to detect light resulting from illumination of the sample by the array(s). One method includes illuminating a microsphere at different positions along a flow path of the microsphere. The method also includes detecting light resulting from the illumination to produce individual output signals corresponding to the illumination at the different positions. The method further includes combining the individual OUTPUT signals to produce a single output signal having a signal-to-noise ratio that is greater than a signal-to-noise ratio of the individual output signals.

Abstract translation: 提供了各种基于发光二极管（LED）的测量系统和方法。 一个系统包括沿着样品（10）的流动路径（20）布置的一个或多个LED阵列（14,16,18）。 阵列被配置为当样品沿着流动路径移动时照亮样品。 该系统还包括一个或多个检测器（24），其被配置为检测由阵列照射样品产生的光。 一种方法包括沿着微球的流动路径在不同位置照射微球。 该方法还包括检测由照明产生的光以产生对应于不同位置处的照明的各个输出信号。 该方法还包括组合各个OUTPUT信号以产生具有大于各个输出信号的信噪比的信噪比的单个输出信号。

公开(公告)号：WO2005017499A3

公开(公告)日：2005-08-18

申请号：PCT/US2004026225

申请日：2004-08-13

Applicant: LUMINEX CORP ,  ROTH WAYNE D ,  MOORE DOUGLAS E

Inventor： ROTH WAYNE D ,  MOORE DOUGLAS E

IPC: G01N15/14

CPC classification number: G01N15/1404 ,  G01N15/1012 ,  G01N21/645 ,  G01N2015/1415 ,  G01N2015/1477 ,  G01N2201/12746

Abstract: Various methods for controlling one or more parameters of a flow cytometer type measurement system are provided. One embodiment includes monitoring parameter(s) of the measurement system during measurements of sample microspheres. The method also includes altering the parameter(s) in real time based on the monitoring. Another method includes monitoring a temperature proximate to the measurement system. One such method includes altering a bias voltage of an avalanche photo diode in response to the temperature using empirically derived data. A different such method includes altering output signals of a photomultiplier tube in response to the temperature using a characteristic curve. Some methods include monitoring a temperature of a fluid, in which sample microspheres are disposed, that will flow through the flow cytometer type measurement system. This method also includes determining a velocity of the sample microspheres in the measurement system from a viscosity of the fluid at the temperature.

Abstract translation: 提供了用于控制流式细胞仪型测量系统的一个或多个参数的各种方法。 一个实施例包括在样品微球的测量期间监测测量系统的参数。 该方法还包括基于监视实时更改参数。 另一种方法包括监测靠近测量系统的温度。 一种这样的方法包括使用经验导出的数据来响应于温度来改变雪崩光电二极管的偏置电压。 不同的这种方法包括使用特性曲线响应于温度改变光电倍增管的输出信号。 一些方法包括监测将流过流式细胞仪类型测量系统的流体样品微球的设置温度。 该方法还包括从该温度下的流体的粘度确定测量系统中的样品微球的速度。

公开(公告)号：WO2010009424A3

公开(公告)日：2010-04-01

申请号：PCT/US2009051039

申请日：2009-07-17

Applicant: LUMINEX CORP ,  ROTH WAYNE D

Inventor： ROTH WAYNE D

IPC: G06F17/00 ,  G01N35/00

CPC classification number: G06K9/00147

Abstract: Methods and systems are provided which include configurations for the reassigning unit locations of a classification matrix at which two or more classification regions overlap as non-classification regions. In addition, methods and systems are provided which include configurations for mathematically creating classification regions which may be characterized by values which more accurately correspond to measured values of particles. Other embodiments of methods and systems include configurations for acquiring data corresponding to measurable parameters of a particle and identifying a location within a classification matrix to which at least some of the data corresponds. Such methods and systems further include configurations for translating either the data corresponding to the identified unit location or a target space located at known locations within the classification matrix a preset number of predetermined coordinate paths until a conclusion that the particle may be classified to particular particle category or a reject class is attained.

Abstract translation: 提供了包括用于两个或多个分类区域作为非分类区域重叠的分类矩阵的重新分配单元位置的配置的方法和系统。 此外，提供了方法和系统，其包括用于数学地创建分类区域的配置，其可以通过更精确地对应于颗粒的测量值的值来表征。 方法和系统的其他实施例包括用于获取对应于粒子的可测量参数的数据的配置，并且识别至少部分数据对应的分类矩阵内的位置。 这样的方法和系统还包括用于将对应于所识别的单元位置的数据或位于分类矩阵内的已知位置处的预定数量的预定坐标路径的目标空间进行翻译的配置，直到可以将粒子分类为特定粒子类别 或获得拒绝类。

公开(公告)号：WO2007143615B1

公开(公告)日：2008-05-08

申请号：PCT/US2007070345

申请日：2007-06-04

Applicant: LUMINEX CORP ,  ROTH WAYNE D ,  COLLINS CHARLES J ,  DUONG DUNG

Inventor： ROTH WAYNE D ,  COLLINS CHARLES J ,  DUONG DUNG

IPC: G01N33/543

CPC classification number: G01N33/54333

Abstract: Systems and methods for performing measurements of one or more materials are provided. One system is configured to transfer one or more materials to an imaging volume of a measurement device from one or more storage vessels. Another system is configured to image one or more materials in an imaging volume of a measurement device. An additional system is configured to substantially immobilize one or more materials in an imaging volume of a measurement device. A further system is configured to transfer one or more materials to an imaging volume of a measurement device from one or more storage vessels, to image the one or more materials in the imaging volume, to substantially immobilize the one or more materials in the imaging volume, or some combination thereof.

Abstract translation: 提供了用于执行一种或多种材料的测量的系统和方法。 一个系统被配置为将一种或多种材料从一个或多个存储容器传送到测量装置的成像体积。 另一系统被配置成对测量装置的成像体积中的一种或多种材料进行成像。 附加系统被配置为基本上将一种或多种材料固定在测量装置的成像体积中。 另一系统被配置成将一种或多种材料从一个或多个存储容器传送到测量装置的成像体积，以对成像体积中的一种或多种材料进行成像，以使成像体积中的一种或多种材料基本上固定 ，或其某些组合。