公开(公告)号：KR1020070024973A

公开(公告)日：2007-03-08

申请号：KR1020050080659

申请日：2005-08-31

Applicant: 대구한의대학교산학협력단

Inventor： 서정철 ,  임강현 ,  정주호 ,  신동훈 ,  최강덕 ,  양재하 ,  김상찬 ,  정승기 ,  이상훈 ,  김용석

IPC: C12Q1/68

CPC classification number: C12Q1/683 ,  C12Q1/6827

Abstract: A kit for discriminating genetic identification among Cervus species through restriction fragment length polymorphism(RFLP) method is provided to supply accurate Cervus sample and be utilized for managing the quality of the Cervus. The method for discriminating genetic identification among Cervus species comprises the steps of: (a) extracting DNA from a Cervus sample; (b) preparing a reaction solution including the extracted DNA, a sense or an anti-sense primer; (c) amplifying the reaction solution; and (d) performing a RFLP on the amplified DNA to genotype it. The discrimination kit analyzes the genetic variation among Cervus species through the RFLP method including a PCR reagent containing a DNA extract reagent, a sense primer or an anti-sense primer and a restriction enzyme.

Abstract translation: 提供了一种通过限制性片段长度多态性（RFLP）方法鉴别鹿种的遗传鉴定的试剂盒，用于提供精确的鹿鹿样品，并用于管理鹿的品质。 用于区分种子遗传识别的方法包括以下步骤：（a）从鹿茸样品中提取DNA; （b）制备包括提取的DNA，有义或反义引物的反应溶液; （c）扩增反应溶液; 和（d）对扩增的DNA进行RFLP以对其进行基因型分析。 鉴别试剂盒通过包括DNA提取试剂，有义引物或反义引物和限制酶的PCR试剂在内的RFLP方法分析鹿茸种间的遗传变异。

公开(公告)号：KR100673070B1

公开(公告)日：2007-01-22

申请号：KR1020050080937

申请日：2005-08-31

Applicant: 대구한의대학교산학협력단

Inventor： 서정철 ,  임강현 ,  정승기 ,  정주호 ,  신동훈 ,  최강덕 ,  양재하 ,  김상찬 ,  이상훈 ,  김용석

IPC: C12Q1/68

CPC classification number: C12Q1/6827 ,  C12Q1/686 ,  C12Q2561/113

Abstract: A method for discriminating genetic identification of ginseng species is provided to be able to supply accurate ginseng raw material through determination of the ginseng species, thereby being used for maintaining quality of the ginseng. The method comprises the steps of: (a) extracting DNA from ginseng sample; (b) method for preparing a reaction solution of primer consisting of the extracted DNA, sense primer(CCAAGTTGCA AACCCATGGT) and anti-sense primer(TTTGATTTCC TTGGCGCATT CC), VIC-primer(VIC-5'TTGGGTGGAT CTCGT-3-MGB) where TagMan MGB probe is attached at 3-terminal of the VIC primer, and FAM-primer(FAM-5'-TTGGGTGGCT CTCGT-3'-MGB) where TagMan MGB probe is attached at 3-terminal of the FAM-primer; (c) amplifying the reaction solution through PCR; (d) performing a real time PCR on the amplified DNA in combination and then analyzing it by comparing a DNA of Panax ginseng binding to the VIC primer obtained from the step(b) and having A nucleotide with a DNA of Panax quinquefolia binding to the FAM primer obtained from the step(b) and having C nucleotide through gene analysis shown in Fig. 1 using an SDS 7000 software so as to determine the Panax ginseng and the Panax quinquefolia.

Abstract translation: 提供了一种辨别人参物种遗传鉴定的方法，可以通过测定人参种类来提供准确的人参原料，从而保持人参的质量。 该方法包括以下步骤：（a）从人参样品中提取DNA; （b）制备由提取的DNA，有义引物（CCAAGTTGCA AACCCATGGT）和反义引物（TTTGATTTCC TTGGCGCATT CC），VIC引物（VIC-5'TTGGGTGGAT CTCGT-3-MGB）组成的引物的反应溶液的方法，其中 TagMan MGB探针连接在VIC引物的3个末端，以及FAM引物（FAM-5'-TTGGGTGGCT CTCGT-3-MGB），其中TagMan MGB探针连接在FAM引物的3个末端; （c）通过PCR扩增反应溶液; （d）组合对经扩增的DNA进行实时PCR，然后通过比较人参参与与步骤（b）获得的VIC引物结合的具有A核苷酸的DNA与DNA结合的Panax quinquefolia进行分析 从步骤（b）获得并具有图8所示的通过基因分析的C核苷酸的FAM引物。 1使用SDS 7000软件，以确定人参和Panax quinquefolia。

公开(公告)号：KR100765412B1

公开(公告)日：2007-10-09

申请号：KR1020050080659

申请日：2005-08-31

Applicant: 대구한의대학교산학협력단

Inventor： 서정철 ,  임강현 ,  정주호 ,  신동훈 ,  최강덕 ,  양재하 ,  김상찬 ,  정승기 ,  이상훈 ,  김용석

IPC: C12Q1/68

Abstract: 본 발명은 제한효소절편길이다형성 (Restriction Fragment Length Polymorphism; RFLP)법에 의한 녹용의 종간 유전자 감별 키트에 관한 것으로써, 상세하게는 제한효소 절편 길이 다형성 법에 의하여 매화록 (
Cervus
Nippon Temminck), 마록 (
Cervus

elaphus ), 대록 (
Cervus

canadensis ) 및 순록 (
Rangifer

Tarandus )의 유전자형을 분석하고, 녹용의 종간 특이적 제한효소를 이용하여 녹용의 종간 연관성과 유전적 변이를 평가하기 위한 감별 키트에 관한 것이다. 본 발명의 감별 키트를 통하여 녹용의 종을 판별함으로써 정확한 녹용 원료를 공급할 수 있으며, 녹용의 품질관리 방법으로 활용할 수 있다.
녹용, 매화록, 마록, 대록, 순록, 제한효소 절편 길이 다형성, 감별 키트

公开(公告)号：KR100673069B1

公开(公告)日：2007-01-22

申请号：KR1020050080936

申请日：2005-08-31

Applicant: 대구한의대학교산학협력단

Inventor： 서정철 ,  임강현 ,  최호영 ,  정주호 ,  신동훈 ,  최강덕 ,  양재하 ,  김상찬 ,  이상훈 ,  김용석

IPC: C12Q1/68

CPC classification number: C12Q1/6827 ,  C12Q1/6809 ,  C12Q1/6869

Abstract: A method for discriminating genetic identification of Saposhnikovia divaricata(TURCZ.) SCHISKIN is provided to supply accurate Saposhnikovia divaricata(TURCZ.) SCHISKIN raw material by discriminating the species of the Saposhnikovia divaricata(TURCZ.) SCHISKIN through a discrimination kit and be utilized for maintaining the quality of Saposhnikovia divaricata(TURCZ.) SCHISKIN. The method comprises the steps of: (a) extracting DNA from Saposhnikovia divaricata(TURCZ.) SCHISKIN; (b) preparing a reaction solution of primer consisting of the extracted DNA, sense primer(CTAACACGTC AACAATTTGG) and anti-sense primer(ACGGACGTAC AATTCAGTTT); (c) amplifying the reaction solution through PCR; (d) analyzing the total nucleotide sequence of the amplified DNA; and (e) comparing a gene obtained by performing a pyrosequencing technique on the amplified DNA in combination with sequences: AGGGTTCGCAGACAGG, Saposhnikovia divaricata(TURCZ.) SCHISKIN, and sequence: AGGATTCGCAGACAGG, Peucedanum japonicum Thunberg to discriminate the Saposhnikovia divaricata(TURCZ.) SCHISKIN showing a G peak in the 4th nucleotide and Peucedanum japonicum Thunberg showing an A peak in the 4th nucleotide.

Abstract translation: 提供了一种鉴别Saposhnikovia divaricata（TURCZ。）SCHISKIN的遗传识别方法，通过鉴别试剂盒鉴别Saposhnikovia divaricata（TURCZ。）SCHISKIN的种类，提供准确的Saposhnikovia divaricata（TURCZ。）SCHISKIN原料，并用于维护 沙棘属（TURCZ。）SCHISKIN的品质。 该方法包括以下步骤：（a）从山茱萸（TURCZ。）SCHISKIN提取DNA; （b）制备由提取的DNA，有义引物（CTAACACGTC AACAATTTGG）和反义引物（ACGGACGTAC AATTCAGTTT）组成的引物的反应溶液; （c）通过PCR扩增反应溶液; （d）分析扩增的DNA的总核苷酸序列; 和（e）将通过对扩增的DNA进行热解测序技术获得的基因与序列组合：AGGGTTCGCAGACAGG，Saposhnikovia divaricata（TURCZ。）SCHISKIN和序列：AGGATTCGCAGACAGG，Peucedanum japonicum Thunberg，以鉴别Saposhnikovia divaricata（TURCZ。）SCHISKIN 在第4核苷酸和日本豌豆um um un showing showing。。。。。。。。。。。。